大花萱草组培苗的练苗技术

大花萱草幼嫩花梗切片接种于MS+6-BA 3.0～5.0mg/L+NAA 0.4～0.8 mg/L的培养基上诱导丛生芽,将丛生芽分割进行增殖培养和生根培养,根系长度3～5cm时在不同的练苗基质上进行驯化练苗。结果表明:基质配比①草炭土:珍珠岩=2:1,②草炭土:珍珠岩:沙土=1:1:1,③草炭土:沙土=1:1的3个基质组合均发根良好,成活率在95%以上,特别是③组合,沙土代替珍珠岩,既免去了冲洗珍珠岩的麻烦,减少珍珠岩粉尘的污染,又降低了成本。同时介绍了组培苗的常规练苗、冬季室内练苗、簇生苗练苗技术,以期为大花萱草组培苗的大面积推广提供理论依据。

Study on Transplant Technique of Tissue Cultured Seedling of Hemerocailis middendorffi

The tender pedicel slice of Hemerocallis middendorffi was inoculated in the substrate of MS+6-BA 3.0～5.0 mg/L+ NAA 0.4～0.8 mg/L for inducing caespitosa shoots.Caespitosa shoots were cut and cultivated for propagation and radication,then acclimatization and transplant were done in different mediums when the length of roots was 3～5 cm.As mediums influence the survival of transplants,different proportioning was designed to obtain those mediums with lower cost and higher survival rate.The results showed that①turfy soil:pe(?)lite=2:1,②tuify soil: perlite:sand=1:1:1,and③turfy soil:sand=1:1.The experiment showed the roots were growing well in above proportioning of mediums and more than 95%survived.Especially in proportioning③,there was no trouble of washing perlite and dust pollution,and save the costs as well.The transplants techniques for conventional tissue culture,winter and caespitosa shoots were introduced at the same time so as to provide theoretical basis for popularizing Hemerocallis plantlets.