| 西洋参发根器官发生及再生植株研究 |
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| 引用本文： | 谢海云,王义,李维,刘伟灿,孙春玉,蒋世翠,张美萍. 西洋参发根器官发生及再生植株研究[J]. 北方园艺, 2011, 0(10): 164-166 |
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| 作者姓名： | 谢海云 王义 李维 刘伟灿 孙春玉 蒋世翠 张美萍 |
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| 作者单位： | 吉林农业大学,生命科学学院,吉林,长春,130118;吉林农业大学,生命科学学院,吉林,长春,130118;吉林农业大学,生命科学学院,吉林,长春,130118;吉林农业大学,生命科学学院,吉林,长春,130118;吉林农业大学,生命科学学院,吉林,长春,130118;吉林农业大学,生命科学学院,吉林,长春,130118;吉林农业大学,生命科学学院,吉林,长春,130118 |
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| 摘 要： | 以西洋参播种苗切断儿为外植体,由发根农杆菌R1601诱导发状根,通过器官发生途径建立高频再生体系,建立西洋参悟性快速繁殖体系。结果表明:诱导西洋参发根脱分化成愈伤组织的最佳培养基为:MS+2,4-D 2.0mg/L+KT 0.5mg/L诱导不定芽增殖和分化的培养基为MS+KT 0.2mg/L+IBA 0.5mg/L诱导生根的培养基为1/2MS+GA3 5.0mg/L+IBA0.5mg/L+NAA 2.0mg/L。
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| 关 键 词： | 西洋参 发根农杆菌 R1601转化 植株再生 |
Study on Organogenesis and Plant Regeneration of Hairy Roots in Panax quinquefolium L. |
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| XIE Hai-yun,WANG Yi,Li Wei,LIU Wei-can,SUN Chun-yu,JIANG Shi-cui,ZHANG Mei-ping. Study on Organogenesis and Plant Regeneration of Hairy Roots in Panax quinquefolium L.[J]. Northern Horticulture, 2011, 0(10): 164-166 |
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| Authors: | XIE Hai-yun WANG Yi Li Wei LIU Wei-can SUN Chun-yu JIANG Shi-cui ZHANG Mei-ping |
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| Affiliation: | XIE Hai-yun,WANG Yi,LI Wei,LIU Wei-can,SUN Chun-yu,JIANG Shi-cui,ZHANG Mei-ping (College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Took sowed the seeds of Panax quinque folium L.as material,then got the little plants,cut the little plants into segments,deal the segments with Agrobacterium rhizogenes R1601,got the hairy roots of Panax quinque folium L. Lastly,obtained the regenerative plants from hairy roots of Panax quinque folium L.by organogenesis,to satablish the efficient transformation system and regeneration methods for transgenic research.The results showed that the best medium from hairy roots to callus was that MS+2,4-D 2.0 mg... |
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| Keywords: | Panax quinquefolium L. Agrobacterium rhizogenes R1601 transformation plant regeneration |
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