| 雏菊组织培养及快速繁殖研究 |
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| 引用本文： | 杨微,贾宝林,钟程,徐娜,姜长阳.雏菊组织培养及快速繁殖研究[J].北方园艺,2011(12):110-112. |
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| 作者姓名： | 杨微 贾宝林 钟程 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029;辽宁师范大学生命科学学院,辽宁,大连,116029;辽宁师范大学生命科学学院,辽宁,大连,116029;辽宁师范大学生命科学学院,辽宁,大连,116029;辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目(20090304); 辽宁师范大学教学改革资助项目(LSJG:20090108) |
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| 摘 要： | 以雏菊的根茎为试材,进行了根茎芽诱导和分化、分化芽的继代和生根、试管苗的移栽和移植的研究,建立起快速繁殖体系。结果表明:MS+6-BA 0.2 mg/L+GA30.5 mg/L+IAA0.2～0.5 mg/L是根茎诱导芽的理想培养基;MS+6-BA 1.0 mg/L+GA30.5 mg/L+NAA 0.1mg/L是根茎诱导芽分化培养和分化增殖培养的理想培养基;1/2MS+NAA 0.1 mg/L+IAA 0.4mg/L是分化芽生根培养的理想培养基;在温室中试管苗的移栽成活率为93.6%,移植到花坛上的试管苗保持了生长旺盛的优良性状。
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| 关 键 词： | 雏菊 组织培养 快速繁殖 |
Study on Tissue Culture and Rapid Propagation of Bellis perennis |
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| YANG Wei,JIA Bao-lin,ZHONG Cheng,XU Na,JIANG Chang-yang.Study on Tissue Culture and Rapid Propagation of Bellis perennis[J].Northern Horticulture,2011(12):110-112. |
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| Authors: | YANG Wei JIA Bao-lin ZHONG Cheng XU Na JIANG Chang-yang |
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| Institution: | YANG Wei,JIA Bao-lin,ZHONG Cheng,XU Na,JIANG Chang-yang(College of Life Science,Liaoning Normal University,Dalian,Liaoning 116029) |
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| Abstract: | In the experiment,the rhizome of Bellis perennis were used as material to do the research on adventitious buds induction and differentiation from rhizome,subculture and rooting of adventitious buds,transplanting of tube seedling,finally establish the rapid propagation system of Bellis perennis.The results showed that the optimum medium for adventitious buds induction and differentiation was MS+6-BA 0.2 mg/L+GA3 0.5 mg/L+IAA 0.2~0.5 mg/L and MS+6-BA 1.0 mg/L+GA3 0.5 mg/L+NAA 0.1 mg/L respectively.1/2MS+NAA 0... |
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| Keywords: | Bellis perennis culture of tissue rapid propagation |
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