| 五种新品种多倍体萱草离体快繁技术研究 |
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| 引用本文： | 宋雪莲,董然,赵和祥,顾德峰.五种新品种多倍体萱草离体快繁技术研究[J].北方园艺,2011(7):127-129. |
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| 作者姓名： | 宋雪莲 董然 赵和祥 顾德峰 |
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| 作者单位： | 吉林农业大学,园艺学院,吉林,长春,130118;吉林农业大学,园艺学院,吉林,长春,130118;吉林农业大学,园艺学院,吉林,长春,130118;吉林农业大学,园艺学院,吉林,长春,130118 |
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| 基金项目： | 吉林省科技厅重点资助项目(20100259) |
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| 摘 要： | 以5个新品种多倍体萱草的幼嫩花茎分蘖的节间为外植体,成功建立了5个品种的快繁体系。结果表明:愈伤诱导最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+2.4-D0.5 mg/L;在增殖培养中,品种"健壮利兰"最佳增殖培养基为MS+6-BA 4 mg/L+NAA 0.4mg/L,增殖倍数为3.12;"浅紫荷"、"Pink Embers"最适增殖培养基为MS+6-BA 5 mg/L+NAA0.5 mg/L,增殖倍数分别为2.93、3.02;"耀辉"、"Bed of Roses"最佳增殖培养基为MS+6-BA2 mg/L+NAA 0.2 mg/L,增殖倍数分别为3、2.87。培养基1/2MS+NAA 0.4 mg/L为"健壮利兰"与"耀辉"最适生根培养基,生根率为100%;"浅紫荷"、"Pink Embers"在1/2MS+NAA 0.5mg/L培养基中生根率达到100%;"Bed of Roses"在1/2MS+NAA 0.6 mg/L培养基中生根率也达到100%。其移栽成活率为100%。
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| 关 键 词： | 多倍体萱草 新品种 快速繁殖 |
Research on Rapid Propagation Technique of Hemerocallis hybrida Polyploid of 5 New Species |
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| SONG Xue-lian,DONG Ran,ZHAO He-xiang,GU De-feng.Research on Rapid Propagation Technique of Hemerocallis hybrida Polyploid of 5 New Species[J].Northern Horticulture,2011(7):127-129. |
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| Authors: | SONG Xue-lian DONG Ran ZHAO He-xiang GU De-feng |
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| Institution: | SONG Xue-lian,DONG Ran,ZHAO He-xiang,GU De-feng(College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | The internode of tillering scape immature of Hemerocallis hybrida polyploid of 5 new species as explants,the successful establishment of five species of rapid propagation system.The results showed that the best medium for callus induction was MS +6-BA 0.5 mg/L+NAA 0.1 mg/L+2,4-D 0.5 mg/L,in the proliferation culture,the best proliferation medium for 'Jianzhuanglilan MS+6-BA 4 mg/L+NAA 0.4 mg/L,the proliferation of multiple was 3.12;'Qianzihe','Pink Embers' the best proliferation of multiple for MS +6-BA 5mg... |
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| Keywords: | Hemerocallis hybrida new species rapid propagation |
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