丝兰皂甙对绵羊瘤胃发酵及血液生化指标的影响

选用12只装有永久性瘤胃瘘管的成年雄性东北细毛羊，采用随机区组试验设计，分别饲喂0、100、200、300mg／kg水平丝兰皂甙，研究其对瘤胃发酵及血液生化指标的影响。对试羊采食后的0、2、4、6、8h瘤胃内环境指标进行测定，各组在同一时间点pH无显著差异（P〉0．05），但总体上随着丝兰皂甙添加水平的上升，pH随时间变化有变缓趋势；300mg／kg组较对照组4h和6h乙酸浓度下降15．1％和19．8％，丙酸浓度则提高22．7％和22．9％，差异显著（P〈0．05），各组总挥发性脂肪酸浓度差异不显著（P〉0．05）；100、200、300mg／kg组对氨的抑制率分别为17．17％、29．84％和27．12％，200mg／kg组较对照组差异显著（P〈0．05）；28d后，测定试羊血液生化指标，各组均处正常生理范围内且差异不显著（P〉0．05）。

Effects of Sarsa-saponine on Rumen Fermentation and Blood Biochemical Traits in Sheep

Twelve male adult local sheep with permanent rumen cannula were used to study the effect of four levels of Sarsa-saponine (0,100,200,300 mg/kg)on rumen fermentation parameters and blood biochemical traits in a completely randomized block design. The pH,VFA and NH_3 concentration of rumen liquid were sampled at 0,2,4,6 and 8 h after feeding. The result showed that pH were not distinctly influenced at the same time points in each group(P>0.05). The variety current of pH have delayed by concentration of Sarsa-saponine supplement increased ;the current of acetic concentration was decreased in each group,but the propionic acids concentration was increased. 300 mg/kg group compared with control ,the acetic concentration decreased by 15.1% and 19.8% , the propionic acids concentration increased by 22.7% and 22.9%,after feeding 4 h and 6 h(P<0.05).The groups showed no difference of the volatile fatty acids concentration(P>0.05);The inhibitory rate of ammonia concentration of 100,200,300 mg/kg group were 17.17%29.84% and 27.12%,and the 200 mg/kg group were distinctly difference compared with control(P<0.05).The result showed that the blood biochemical traits of four groups were in normal range and were not distinctly varied(P>0.05)after feeding 28 d.