Plus tree of Cryptomeria japonica D. Don with a heterozygous male-sterility gene |
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Authors: | Maki Saito Hideaki Taira |
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Institution: | (1) Forest Experiment Station, Toyama Forestry and Forest Products Research Center, 3 Yoshimine, Tateyama-machi, Nakaniikawa-gun, Toyama 930-1362, Japan;(2) Niigata University Graduate School of Science and Technology, Biosphere Course, Niigata, Japan |
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Abstract: | To find plus tree clones of Cryptomeria japonica that are heterozygous for a male-sterility gene (Aa), we crossed a homozygous male-sterile tree (aa) with 63 clones. Male sterility in this case is controlled by a recessive allele at a single gene locus and is expressed only in homozygotes. All F1 seedlings obtained by crossing the male-sterile mother tree and 62 out of the 63 clones produced pollen. In contrast, F1 seedlings obtained from the crossing between the male-sterile mother tree and a plus tree clone, Ohara 13, produced 64 male-sterile individuals and 52 fertile individuals. The segregation ratio fitted the expected 1 : 1 ratio according to a chi-square test. These results clearly demonstrate that the Ohara 13 clone is heterozygous for a male-sterility gene. |
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Keywords: | Allergic disease Cryptomeria japonica D Don Male-sterility gene Plus tree |
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