Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.) |
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| Authors: | Kyung-Ho Ma Jae-Gyun Kwag Weiguo Zhao Anupam Dixit Gi-An Lee Haeng-Hoon Kim Ill-Min Chung Nam-Soo Kim Jae-Sun Lee Jae-Jun Ji Tae-San Kim Yong-Jin Park |
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| Affiliation: | 1. Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA 225, Seodun-Dong, Suwon 441-707, Republic of Korea;2. Department of Applied Bioscience, Konkuk University, 1 Hwayang-dong, Seoul 143-701, Republic of Korea;3. Rice DNA and Quality Testing Laboratory, Basmati Export Development Foundation, SVBP University of Agriculture and Technology, Meerut 250110, India;4. Department of Molecular Bio-Sciences, Kangwon National University, 192-1 Hyoja-Dong, Chuncheon 200-701, Republic of Korea;5. Garlic Research Institute, Agriculture Research and Extension Service, 659-1, Danyang 395-841, Republic of Korea;6. Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan 340-802, Republic of Korea |
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| Abstract: | Here we characterized eight novel polymorphic SSR markers, developed from an enriched genomic library of garlic (Allium sativum L.). These SSRs produced a total of 64 alleles across 90 garlic accessions, with an average of 8 alleles per locus. Values for observed (HO) and expected (HE) heterozygosity ranged from 0.16 to 0.77 (mean = 0.44) and from 0.22 to 0.86 (mean = 0.65), respectively. Six loci deviated significantly (P < 0.05) from Hardy–Weinberg equilibrium (HWE). The averages of gene diversity and PIC values were 0.65 and 0.62, respectively. The mean genetic similarity coefficient was 0.4380, indicating that among garlic accessions existed wide genetic variation. Based on 64 alleles obtained by 8 SSRs, a phenogram was constructed to understand the relationships among the 90 accessions. These newly developed SSRs should prove very useful tools for genotypes identification, assessment of genetic diversity and population structure in garlic. |
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| Keywords: | Allium sativum L. Garlic Genetic diversity SSRs |
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