高效液相色谱法测定桑枝黑木耳中 DNJ 的含量

通过柱前衍生化高效液相色谱法测定桑枝黑木耳中1-脱氧野尻霉素(1-deoxynojimycin ,DNJ)的含量.结果表明 ,用芴甲氧酰氯(FMOC-Cl)为衍生化试剂衍生化DNJ ,选择Waters X-bridge C18色谱柱 ,流动相为乙腈-0 .1% 醋酸(体积比55︰45) ,流速为1 .0 mL/min ,UV检测波长为254nm时 ,DNJ-FMOC 的峰面积与DNJ浓度呈高度正相关 ,相关系数为0 .9966 ,该检测方法灵敏、准确、稳定的、快捷 ,适合桑枝黑木耳等桑枝食用菌中 DNJ含量的检测.普通黑木耳不能自我合成DNJ ,但对桑枝中的DNJ有较好的富集能力 ,桑枝黑木耳中DNJ含量高达0 .3854% ,可利用桑枝黑木耳等桑枝食用菌对DNJ的富集能力来提高桑枝食用菌的开发利用价值.

Determination of DNJ in Black Fungus Planted on Mulberry Branches by High Performance Liquid Chromatography

Pre-column derivatization high performance liquid chromatography (HPLC) was applied to determine the content of 1-deoxynojimycin (DNJ) in black fungus planted on mulberry branches .The results showed that when the DNJ marked with 9-fluorenyl methyl chloroformate was detected with WatersX-bridge C18 column ,the mobilephase consisted of aceto-nitrile-0 .1% aqueous acetic acid (55︰45) ,the rate of 1 .0mL/min ,the excitation wave length of 254nm ,and there was a high positive correlation between the peak area and concentration of DNJ-FMOC with a correlation coefficient of 0 .9966 . This method is sensitive ,accurate ,stabilized ,rapid and applicable for the determination of DNJ in black fungus planted on mulberry branches .The common black fungus could not synthesize DNJ by itself ,but it could enrich DNJ from mul-berry branches ,and the content of DNJ in black fungus on mulberry branches was up to 0 .3854% .In the future ,we can cultivate edible fungi with stronger enrichment ability of DNJ by mulberry branches to improve development and utilization of mulberry branches black fungus .