纳米硒对岢岚绒山羊及胎儿的生物学效应

为研究纳米硒对岢岚绒山羊妊娠母羊及胎儿机体免疫力、抗氧化性和生长发育的影响,选择体重相近的岢岚妊娠绒山羊80只,随机分为2组,分别喂以基础日粮(C组)和添加0.5mg/kgDM纳米硒的饲粮(Se组),实验期110d。结果表明:妊娠期绒山羊日粮中添加硒后,血液中淋巴细胞、单核细胞、嗜中性粒细胞的百分比显著提高(p<0.05),异型淋巴细胞百分比显著降低(p<0.05),血清中血清球蛋白(GLO)、免疫球蛋白IgG和IgM显著提高(p<0.05);日粮中纳米硒的添加极显著(p<0.01)提高了母羊及胎儿血清中GSH-Px、SOD的活性,极显著(p<0.01)提高了母羊及胎儿肝、胎盘组织中GSH-Px、SOD的活性,极显著(p<0.01)降低了母羊及胎儿血清与胎盘组织中MDA的浓度;在日粮中添加纳米硒后,显著增加了胎盘、胎儿及胎儿肝、肾组织的重量(p<0.05)。结论:日粮中添加0.5mg/kgDM纳米硒,增强了妊娠母羊及胎儿机体免疫力和抗氧化能力,进一步促进了胎儿的生长发育。

The Biological Effect of Selenium at Nano-size on Pregnant Cashmere Goat and Fetus

The study was conducted to investigate the effects of Nano-Se on immunologic function, antioxidation function, growth and development of pregnant cashmere goat and fetus in KeLan. A total of 80 pregnant cashmere goats in KeLan with approximately equal body weight were allotted to one of two experimental treatments, a control ( C, n=40 ) without supplemental Se in basal diet or supplemented ( Se, n=40 ) with nano-selenium (declared Se content 0.5 mg/kg DM), The whole feeding experiment lasted for 110 days. The results from supplemental Nano-Se in diet showed that S group were significant effect by adding selenium to pregnancy cashmere does on the percentage of Lymphocytes, Monocytes and Neutrophils of immunologic function(P<0.05), The percentage of atypical lymphocyte in S group were significantly lower than C group (P<0.05), GLO、IgG and IgM of S group was significantly higher than C group (P<0.05). Adding Nano-Se in diet, Activities of glutathione peroxidase (GSH-Px) and superoxide dismutase ( SOD) were markedly increased(p<0.01) in serum of pregnant goats and fetus, activities of GSH-Px and SOD were markedly increased (p<0.01) in live、placental tissues of pregnant goats and fetus, malondialdehyde (MDA) concentration were markedly decreased (p<0.01) in serum of pregnant goats and fetus and placental tissue. Supplementation of basal diet with Nano-Se markedly increased the weight of placenta, fetus, fetal liver and kidney tissues (P<0.05). It is concluded that supplementation of Nano-Se can changed the ratio of immune cells, enhanced immunologic function, improve the antioxidation function, and increase growth development of fetus. Supplementation level of Nano-Se is 0. 5 mg/kg DM in basal diet for pregnant goats.