Type VII and XVII Collagen mRNA Expressions in Regenerated Epidermal Laminae
in Chronic Equine Laminitis |
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Authors: | Atsutoshi KUWANO Telhisa HASEGAWA Katsuhiko ARAI |
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Institution: | 1. Equine Research Institute, Japan Racing Association, 321–4 Tokami-cho, Utsunomiya-shi, Tochigi 320-0856, Japan;2. Faculty of Agriculture Scleroprotein and Leather Research Institute, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan |
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Abstract: | To confirm ability forming the basement membrane of the regenerated laminar epidermis
(rLE) in chronic laminitis, expression of type VII and type XVII collagen mRNAs in the rLE
was studied applying sequences of two type of murine collagens. On northern blot analysis,
complement DNA (cDNA) probes adjusted from the murine type VII and type XVII collagen
could hybridize with the equine mRNAs, and each signal was detected as single-bands at
approximately 9.5 kb and 5.6 kb, respectively. Contrasting with the expression level of
equine glyceraldehyde-3-phosphate dehydrogenease mRNA, the band of type VII collagen mRNA
in laminitis was stronger than normal, but the type XVII collagen mRNA in laminitis was
less than normal. By in situ hybridization, positive signals in response to the murine
type VII and type XVII collagen mRNA probes could be detected in the equine laminitic rLE
region. From these results, it is concluded that the keratinocytes constructing the rLE in
chronic stage of laminitis can express type VII and type XVII collagen mRNAs and these
expression patterns were different from the normal. |
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Keywords: | equine laminitis type VII collagen type XVII collagen |
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