NaCl胁迫对紫叶李(Prunus cerasifera var. atropurea)叶片生理生化特性的影响

为研究紫叶李的抗NaCl特性,以盆栽4年生紫叶李为试材,设置土壤的NaCl含量为CK(0.042%),0.1%,0.2%,0.3%,0.4%,研究NaCl胁迫对紫叶李的膜质过氧化程度、抗氧化酶活性、渗透调节物质含量的影响。结果表明:随着土壤中NaCl含量的增高,细胞膜透性增大,丙二醛(MDA)含量增加,氧自由基的产生速率加快;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性变化趋势一致,均为先上升后下降;脯氨酸含量呈增加趋势,可溶性糖含量先增加后减少,可溶性蛋白含量呈下降趋势,说明脯氨酸和可溶性糖可能是盐胁迫下紫叶李的主要渗透调节物质。结合各项生理指标和盐害表现可以得出,紫叶李抗NaCl胁迫的阈值为0.3%土壤含盐量。

Effects of NaCl stress on physiological and biochemical characteristics of leaves of Prunus cerasifera var.atropurea

To study the characteristics of salt resistance of Prunus cerasifera var.atropurea,4-year-old potted Prunus cerasifera var.atropurea treated by NaCl was used.The soil salt contents were CK(0.042%),0.1%,0.2%,0.3% and 0.4% respectively.The effects of NaCl stress on membrane lipid peroxidation,antioxidant enzyme activity,and osmotic adjustment substance contents were researched.The results were as follows: With the increase of soil NaCl content,membrane lipid peroxidation,MDA content and the generating rate of free radical increased;SOD,POD and CAT increased first and then decreased with the increase of soil NaCl content;Proline was increased,Soluble sugar increased at first,and then decreased,and Soluble protein decreased,indicating that the Proline and Soluble sugar might be the main osmotic adjustment contents in Prunus cerasifera var.atropurea.Results from the physiological index and performances of salt harm show that the soil salt content 0.3% is the threshold concentration of Prunus cerasifera var.atropurea.