| 从新旧蚯蚓匀浆液中制备蚯蚓纤溶酶组分的比较研究 |
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| 引用本文： | 刘泽民,赵晓瑜,刘彬彬,李冬,张锋,张冬,崔大岭.从新旧蚯蚓匀浆液中制备蚯蚓纤溶酶组分的比较研究[J].河北农业大学学报,2006,29(6):95-98. |
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| 作者姓名： | 刘泽民 赵晓瑜 刘彬彬 李冬 张锋 张冬 崔大岭 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北省重点学科建设项目 |
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| 摘 要： | 为探寻从蚯蚓匀浆液中制备蚯蚓纤溶酶(EFE)的简便途径,利用亲和层析的方法,分别从新鲜制备的与多年存放的蚯蚓匀浆液中制备了新、旧蚯蚓纤溶酶(即新、旧EFE)。利用天然电泳、SDS-PAGE纤维蛋白活性电泳分析组分差异,通过纤维蛋白平板比较两者纤溶酶活性,发现蚯蚓匀浆液在存放过程中,EFE的部分组分发生降解,但EFE的比活却升高了1.4倍。
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| 关 键 词： | 蚯蚓 纤溶酶 亲和层析 组分 |
| 文章编号： | 1000-1573（2006）06-0095-04 |
| 收稿时间： | 2006-05-20 |
| 修稿时间： | 2006-05-20 |
Comparison of components of fibrinolytic enzymes from new and old earthworm extracts |
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| LIU Ze-min,ZHAO Xiao-yu,LIU Bin-bin,LI Dong,ZHANG Feng,ZHANG Dong,CUI Da-ling.Comparison of components of fibrinolytic enzymes from new and old earthworm extracts[J].Journal of Agricultural University of Hebei,2006,29(6):95-98. |
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| Authors: | LIU Ze-min ZHAO Xiao-yu LIU Bin-bin LI Dong ZHANG Feng ZHANG Dong CUI Da-ling |
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| Institution: | College of Life Science, Hebei University, Haoding 071002, China |
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| Abstract: | The new and old earthworm fibrinolytic enzymes(EFE) were separated from the new and old crude extracts of the earthworm tissue by affinity chromatography.There were differences between the new and old EFE in PAGE,Finrin-SDS-PAGE and fibrinolytic activity.Some components of old EFE were partially degraded, and the fibrinolytic activity of old EFE was 1.4 fold of the new EFE. |
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| Keywords: | earthworm(Eisenia faetida) fibrinolytic enzymes affinity chromatography components |
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