Somatic embryo culture for propagation,artificial seed production,and conservation of sawara cypress (<Emphasis Type="Italic">Chamaecyparis pisifera</Emphasis> Sieb. et Zucc.) |
| |
Authors: | E Maruyama Y Hosoi K Ishii |
| |
Institution: | (1) Transformation Laboratory, Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, PO Box 16, Tsukuba Norinkenkyudanchi-nai, Ibaraki 305-8687, Japan Tel. +81-298-733211; Fax +81-298-731542 e-mail: tsumaruy@ffpri.affrc.go.jp, JP |
| |
Abstract: | Somatic embryogenesis in Chamaecyparis pisifera Sieb. et Zucc. was initiated from immature seeds collected from the end of June to early July. Mass propagation through adventitious
shoot bud production from somatic embryo culture on Woody Plant (WP) medium and artificial seed production using sodium alginate
was achieved. A high bud forming index value (25.8) was obtained on medium supplemented with 1 μM 6-benzylaminopurine. The conversion rates from artificial seeds under aseptic and nonaseptic conditions were 60%–100% and
10%–12%, respectively. For germplasm conservation, somatic embryos and embryogenic cells were successfully stored at 4°C (medium-term
storage) and in liquid nitrogen for long-term storage.
Received: December 21, 2001 / Accepted: August 1, 2002
Acknowledgments This work was supported in part by the Japan Science and Technology Corporation and in part by a Grant for Research for the
Future Program from the Japan Society for the Promotion of Science.
Correspondence to:E. Maruyama |
| |
Keywords: | Chamaecyparis pisifera Somatic embryogenesis Cryopreservation Artificial seeds Embryo culture |
本文献已被 SpringerLink 等数据库收录! |
|