日粮添加维生素E对免疫抑制产蛋鸡经济性状下降的调控效应

 【目的】研究在基础日粮中添加维生素E对环磷酰胺诱导产蛋鸡免疫抑制的调控效应。【方法】将270 只健康尼克褐产蛋鸡随机等分为5组，每组3个重复，每个重复18只。Ⅰ、Ⅱ组饲喂基础日粮（维生素E含量44.59 mg?kg-1），Ⅲ、Ⅳ、Ⅴ组在基础日粮中分别添加50、100和200 mg?kg-1维生素E。试验第5、6、7天，对Ⅱ—Ⅴ组产蛋鸡注射80 mg?kg-1 BW环磷酰胺建立免疫抑制模型，第Ⅰ组注射等量生理盐水。【结果】日粮中添加VE能显著提高免疫抑制产蛋鸡的产蛋性能和饲料转化率、养分表观代谢率（P＜0.05），提高免疫抑制产蛋鸡脾脏和胸腺的相对重量和新城疫（new castle disease，ND）、H5和H9亚型禽流感（avian influenza，AI）抗体滴度及血浆谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、超氧化物歧化酶（superoxide dismutase，SOD）活性（P＜0.05），降低丙二醛（malondialdehyde，MDA）的含量（P＜0.05）；提高免疫抑制产蛋鸡血浆的PGE2、IL-1、IL-6和TNF-α含量（P＜0.05）。【结论】环磷酰胺诱导产蛋鸡所产生的免疫抑制可显著降低其生产性能、养分利用率、抗体水平、抗氧化功能及PGE2和炎性细胞因子含量；日粮中添加维生素E对免疫抑制蛋鸡具有明显的调控效应，且添加水平为50和100 mg?kg-1时效果优于200 mg?kg-1的添加量。

Modulation of Dietry Vitamin E on Economic Characterization of Immunosupressed Laying Hens

【Objective】The modulation of dietary vitamin E levels on immunosuppressed laying hens induced by cyclophosphamide (CTX) was studied. 【Method】Two hundred and seventy Nick Red laying hens were randomly divided into 5 groups and each group had 3 replications with 18 birds. Laying hens from group Ⅲ, Ⅳ, and Ⅴ(birds treated with vitamin E) were fed diets supplemented with 50, 100 and 200 mg?kg-1 vitamin E, respectively, whereas group Ⅰ and Ⅱ received only the corn-soybean meal basal diets with 44.59 mg?kg-1 of vitamin E. Laying hens in group Ⅱ-Ⅳ(birds challenged with CTX) were injected in the thigh muscle with CTX at 80 body weight for 3 days, and that in groupⅠ(control group) were injected with the same amount of sterile saline. 【Result】Supplemental vitamin E significantly increased egg production, feed efficiency and nutrient digestibility (P＜0.05). The relative weights of the spleen and thymus to the body weight, serum HI antibody titers to H5-AI, H9-AI and ND, plasma GSH-Px and SOD activities were greater in immunosuppressed birds fed supplemented vitamin E (P＜0.05), and the MDA concentration was least at 50 mg?kg-1 supplemental vitamin E, and further additions had negative effect (P＜0.05). Birds treated with CTX fed diets with supplemental 100 and 200 mg vitamin E/kg had greater plasma PGE2, IL-1, IL-6, and TNF-α levels (P＜0.05). 【Conclusion】The results showed that dietary vitamin E supplementation significantly attenuated immunosuppressive effect of CTX challenge in laying hens.