核酸水平检测犬副流感病毒方法的建立

根据GenBank中与犬副流感(CPIV)同源性较近的SV5病毒N基因保守序列,利用DNAStar软件设计了一对特异性引物,能扩增265bp大小的片段,并以此建立了检测CPIV的RT-PCR方法,实验证明该对引物特异扩增CPIV;不扩增犬瘟热病毒、犬细小病毒、犬腺病毒和狂犬病病毒犬的四种病原的核酸。检测临床病料20份,其中2分为CPIV阳性。此法敏感性较高。是检测犬急性传染性呼吸道疾病(CIRD)中CPIV的有效的方法。

Detection of Canine Parainfluenza Virus by PCR

A pair of PCR primers were designed according to the nucleocapsid protein sequences of Simian vires 5(SV5)in GenBank by Primer 5.0 software.The product is 265bp fragment.A RT-PCR was constructed method, which can be used to detect canine parainfluenza vires(CPIV).The specific experiment of PCR showed that the primers could only amplify the genome of CPIV.No any gene fragments had been amplified from the cells that were infected by canine distemper virus,canine parvovirus,canine adenovirus and rabies virus.The sensitivy ex- periment showed that the method could amplify the specific fragment from the soliquoid of spleen cell infected by CPIV at 10~(-5)dilution.It showed that this study provides an effective means for chnical rapid diagnosis of CPIV in CIRD.