黑果花楸花色苷提取、纯化工艺研究

以黑果花楸花色苷得率为考察指标,分别用水,50%、60%、70%、80%、90%、100%的乙醇和50%、60%、70%、80%、90%、100%的甲醇作为溶剂提取黑果花楸花色苷,得出80%乙醇为较好的提取溶剂。以80%乙醇为提取溶剂,进行提取时间、料液比、pH值、提取温度4个单因素试验。在单因素试验基础上,运用Box-Behnken响应面设计建立数学模型。结果表明最佳提取条件为:提取时间110 min、pH值2、提取温度34 ℃、料液比1∶16.5,黑果花楸花色苷得率为6.648 mg/g。通过静态吸附和解吸筛选出黑果花楸花色苷的最佳纯化树脂为AB-8,最佳纯化条件为上样质量浓度2 mg/mL,径长比1∶25,用2.2 BV、pH值2的80%乙醇以2.0 BV/h的流速洗脱,可使花色苷纯度提高11.5倍。 

Extraction and purification conditions of anthocyanin from the fruit of Aronia melanocarpa

Anthocyanins were extracted from Aronia melanocarpa with water, 50% -100% ethanol and 50% -100% methanol. The results showed that the optimal yield of anthocyanins was with 80% ethanol. Four single factor experiments were designed for extracting time, solid-liquid ratio, pH and temperature. Based on the single factor experiment analysis, the mathematical model was established by Box-Behnken response surface design. The results showed that the optimum extracting conditions were:extracting time 110 min, pH 2, extracting temperature 34 ℃, solid-liquid ratio 1: 16. 5. Under these conditions, anthocyanins yield reached 6. 648 mg/g. AB-8 was chosen as the optimal resin after the static adsorption and desorption testing. The optimum purification conditions were 2 mg/mL of solution concentration, 1:25 of diameter to length, 2. 2 BV, pH 2 and 80% ethanol with the elution flow rate of 2. 0 BV/h, the anthocyanins purity increased 11. 5 times.