|
|||||
|
|
| AFLP分子标记技术在名贵春兰鉴别中的应用 | |
| 引用本文： | 陈惠云,孙志栋,孙日飞,王晓武,葛 红.AFLP分子标记技术在名贵春兰鉴别中的应用[J].中国农学通报,2007,23(2):70-070. |
| 作者姓名： | 陈惠云 孙志栋 孙日飞 王晓武 葛 红 |
| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,北京,100081;宁波市农科院,宁波,315040 2. 宁波市农科院,宁波,315040 3. 中国农业科学院蔬菜花卉研究所,北京,100081 |
| 基金项目： | 浙江省宁波市科技计划;浙江省宁波市农业科学院院长基金 |
| 摘 要： | 从基因水平建立名贵春兰品种的鉴定方法，突破以往只能用传统方法鉴定名贵春兰品种的局限性；利用AFLP分子标记技术对18种主要名贵春兰品种进行了DNA指纹图谱分析,结果筛选出16对引物，这16对引物在18个名贵春兰品种中共扩增出345条带(平均每对引物扩增21.56条带)，其中有多态性带169条(平均每对引物扩增多态性带10.56条)，多态性比例为48.99%；通过这些多态性条带的不同组合，可明确将供试的18个名贵春兰品种加以区分，并作为18个名贵春兰品种鉴定的依据。 |
| 关 键 词： | 植物雌激素 植物雌激素 槲皮素 非洲爪蟾 体重 畸形 |
| 修稿时间： | 2006-10-172006-10-23 |
AFLP Molecular Markers was Applied in Identification of Precious Varieties of Cymbidium goeringii(Rchb.f)Rchb.f |
|
| Chen Hui yun,Sun Zhi dong,Sun Ri fei,Wang Xiao wu,Ge Hong.AFLP Molecular Markers was Applied in Identification of Precious Varieties of Cymbidium goeringii(Rchb.f)Rchb.f[J].Chinese Agricultural Science Bulletin,2007,23(2):70-070. | |
| Authors: | Chen Hui yun Sun Zhi dong Sun Ri fei Wang Xiao wu Ge Hong |
| Institution: | 1 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing ,0008; 2Ningbo Academy of Agricultural Sciences, Ningbo, 315040 |
| Abstract: | To set up a new identification and analysis method of Cymbidium goeringii(Rchb.f)Rchb.f based on the level of gene has broken through the restriction of the traditional method. AFLP fingerprinting analysis on 18 rare varieties of Cymbidium goeringii(Rchb.f)Rchb.f was carried out .16 primer-pairs were screened out and amplified 345 nucleotide acid fragments in the 18 rare varieties of Cymbidium goeringii(Rchb.f)Rchb.f, which the polymorphism rate was about 48.99 %, the average bands amplified by each primer-pair were 21.6. The different combination of polymorphic bands can distinguish the 18 rare Cymbidium varieties, and can be used to identify 18 rare varieties of Cymbidium goeringii(Rchb.f)Rchb.f. |
| Keywords: | AELP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农学通报》浏览原始摘要信息 | |
| 点击此处可从《中国农学通报》下载免费的PDF全文 | |