| 鸡小肠上皮细胞的分离及体外培养研究进展 |
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| 引用本文： | 王晓亮,顾宪红,郑冬梅. 鸡小肠上皮细胞的分离及体外培养研究进展[J]. 中国畜牧兽医, 2010, 37(9): 34-38 |
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| 作者姓名： | 王晓亮 顾宪红 郑冬梅 |
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| 作者单位： | （中国农业科学院北京畜牧兽医研究所 动物营养学国家重点实验室，北京 100193） |
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| 基金项目： | 国家自然科学基金项目 |
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| 摘 要： | 小肠是食物消化吸收的主要场所,而小肠上皮细胞(intestinal epithelial cells,IEC)在营养物质的消化吸收及转运、消化酶分泌及肠道免疫方面发挥着重要作用。利用体外培养方法分离培养IEC为研究其分化增殖、凋亡等生物学功能提供了重要手段。作者综述了培养组织样的选择、分离IEC的常用方法、影响IEC培养的因素及IEC的鉴定方法等鸡IEC体外分离培养过程中的相关研究进展。
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| 关 键 词： | 小肠上皮细胞 鸡 分离 体外原代培养 |
The Advance on Isolation and Primary Culture of Chicken Intestine Epithelial Cells in vitro |
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| WANG Xiao-liang,GU Xian-hong,ZHENG Dong-mei. The Advance on Isolation and Primary Culture of Chicken Intestine Epithelial Cells in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(9): 34-38 |
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| Authors: | WANG Xiao-liang GU Xian-hong ZHENG Dong-mei |
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| Affiliation: | (State Key Laboratory of Animal Nutrition,Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193,China) |
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| Abstract: | Small intestine is the main place for digestion and absorption of the food, while the intestinal epithelial cells (IEC) play an important role in the digestion, absorption and transport of nutrient, digestive enzyme secretion and intestinal immunity. The isolation and primary culture of IEC provide an important tool to study differentiation, proliferation, apoptosis and other biological functions of the IEC. This article overviewed the relevant progress in culture of chicken IEC in vitro, such as the choice of tissue culture, common method for isolation method of IEC, factors that affect the IEC culture and identification method |
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| Keywords: | intestine epithelial cells chicken isolation primary culture in vitro |
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