瘿蚊科3种昆虫核糖体DNA的克隆及序列分析

瘿蚊科是双翅目昆虫中的重要类群?本研究对瘿蚊科3种昆虫—麦红吸浆虫?菊花瘿蚊和食蚜瘿蚊的核糖体DNA进行PCR扩增?克隆?测序及序列分析, 并对ITS-1在麦红吸浆虫4个地理种群中的遗传变异情况进行分析?结果表明, 从3种昆虫中获得的核糖体DNA序列包括:部分的18S rDNA(44 bp)?28S rDNA(37 bp), 全部的ITS-1(487~535 bp), 5.8S rDNA(121 bp)及ITS-2(336~352 bp)序列?3种昆虫ITS序列的碱基差异百分比在17.21%~29.59%之间, 共含有206个变异位点?ITS-1序列在麦红吸浆虫4个地理种群中比较保守, 只有4个变异位点, 单倍型多样性为0.311 7~0.796 5, 核苷酸多样性为0.000 6~0.002 2?本研究为今后瘿蚊科昆虫的分类鉴定?系统发育和遗传进化等相关研究提供了基础?

Cloning and sequence analysis of ribosomal DNAs from three species of the Cecidomyiidae family

Cecidomyiidae is an important insect group in the order Diptera. The ribosomal DNAs from three gall midges, Sitodiplosis mosellana, Rhopalomyia longicauda and Aphidoletes aphidimyza, were studied by PCR amplification, cloning, sequencing and sequence analysis. The genetic variation of ITS-1 in four geographical populations of S.mosellana was analyzed. The results showed that the rDNA sequences of three insects included partial sequences of 18S rDNA (44 bp) and 28S rDNA (37 bp), complete sequences of ITS-1 (487-535 bp), 5.8 S rDNA (121 bp) and ITS-2 (336-352 bp) sequences. The difference among ITS sequences was between 17.21% and 29.59%, including a total of 206 variant sites. There was little difference in the ITS-1 sequence among the four geographical populations of S.mosellana, with only 4 variant sites, a haplotype diversity of 0.311 7-0.796 5, and a nucleotide diversity of 0.000 6-0.002 2. This study provides a basis for the study of identification, phylogeny and genetic evolution of gall midges of the Cecidomyiidae family.