细胞松弛素B对绵羊GV期卵母细胞玻璃化冷冻效果的影响

本试验通过玻璃化冷冻前细胞松弛素B(CB)预处理来分析CB对绵羊GV期卵母细胞玻璃化冷冻/解冻后发育潜力的影响。分别从细胞毒性检测、冷冻检测和CB不同浓度（6.0、7.5和9.0 μg/ml）处理3个方面进行试验。试验结果表明毒性检验中CB处理组和未处理组卵母细胞的成熟率都显著低于对照组(P<0.05)，但相互之间差异不显著；玻璃化冷冻后，卵母细胞成熟率显著低于未冷冻组(P<0.05)，玻璃化冷冻CB处理组和未处理组卵母细胞体外成熟培养后成熟率之间差异不显著(P>0.05)；不同CB浓度处理后9.0 μg/ml组卵母细胞的成熟率显著高于对照组（P<0.05）。

The Effects of Cytochalasin B on Vitrification of Ovine GV-stage Oocytes

In this experiment, the effect of cytochalasin B (CB) on vitrification of ovine GV-stage oocytes were analyzed. This experiment included toxicity test,vitrification and pre-treatment with different cytochalasin B (CB) concentration. The results were as follows: rates of matured oocytes of toxicity groups both, with or without CB pre-treatment were lower than control group (P<0.05), but there was no difference between them(P>0.05). In vitrified groups, the rate of matured oocytes was 16.1% and 14.1% respectively after in vitro maturation culture, they were all significantly lower than control group (P<0.05). 9.0ug/ml of CB could improve the maturation rate compared with control group(P<0.05).