生长素和细胞分裂素对怀山药微型块茎诱导形成的影响

以B号山药试管苗为材料,研究了生长素NAA,2,4-D和细胞分裂素KT,6-BA单独使用对怀山药微型块茎诱导形成的影响。结果表明,与对照相比,生长素有利于微型块茎的有效数量、总重量、单株平均微型块茎数及块茎指数的显著提高,NAA和2,4-D诱导微型块茎形成的适宜浓度分别为0.5 mg/L和0.1 mg/L,且以2,4-D 0.1 mg/L的诱导效果最好;细胞分裂素对微型块茎的形成不利,其中6-BA效果更差,微型块茎各项指标均无显著提高,且浓度大于2 mg/L时无微型块茎形成。

Effects of Auxin and Cytokinin on the Induction of Microtuber of Disocorea opposita Thunb.

The individual effects of NAA,2,4-D,KT and 6-BA on the microtuberization of Disocorea opposita Thunb.cv.B were investigated.The results showed,compared with control,the auxin alone was advantageous to increase the valid number of microtubers,total weight,average number of microtubers per plantlet and microtuber index remarkably.NAA,2,4-D at 0.5 mg/L and 0.1 mg/L respectively showed positive effects on microtuber induction,and 2,4-D at 0.1 mg/L has the best effects on induction of microtuber.The cytokinin was disadvantageous to the formation of microtubers.6-BA treatment had the less effects on every microtuber indexes,furthermore,when the concentration was higher than 2 mg/L,the microtuber indexes didn't increase remarkably and there are no microtuber forming at the high contration.