长枝木霉Trichoderma longibrachiatum T6水分散粒剂的研制

以土壤中分离得到的一株拮抗菌——长枝木霉Trichoderma longibrachiatum T6为研究对象,通过优化培养基配方及助剂类型的筛选,成功研制了长枝木霉T. longibrachiatum T6水分散粒剂,并测定了其贮存稳定性。结果表明:制备长枝木霉T6水分散粒剂的最佳配方(质量分数)为:羧甲基纤维素(紫外保护剂)0.5%,碳酸钙(稳定剂)4%,聚乙烯基吡咯烷酮(PVP,黏结剂)5%,可溶性淀粉(崩解剂)5%,凹凸棒土(载体)5%及十二烷基硫酸钠(润湿剂)5%。由此配方制备的水分散粒剂中孢子含量为5.6×108 cfu/g,悬浮率53%,pH值6.0,水分含量3.5%,湿润时间52 s,且在冷藏温度为(4±2)℃条件下的稳定性显著高于在室温(25℃)下的。各项检测结果均符合国家标准。

Research and development of water dispersible granules of Trichoderma longibrachiatum T6

The water dispersible granules ofTrichoderma longibrachiatum T6 was developed. This antagonistic bacteria was isolated from field soil. TheT. longibrachiatum T6 water dispersible granules were developed by optimizing media formulations and screening the aid types. And the storage stability was measured. The results indicated that the best formulation (mass fraction) was hydroxymethyl cellulose (UV protective agents) 0.5%, calcium carbonate (stabilizing agents) 4%, polyvinylpyrrolidone (PVP, binders) 5%, soluble starch (disintegrating agents) 5%, attapulgite (carriers) 5%, sodium lauryl sulfate(witting agents)5%. The quality detection showed that the spores production ofT. longibrachiatum T6 water dispersible granules was 5.6 × 108 cfu/g, the suspension rate of the production was 53%, pH was 6.0, the moisture amount was 3.5%, the wetting time was 52 s, the stability in low temperature of (4 ± 2)℃ was significantly higher than that at room temperature 25℃, which came up to the formulation standards.