玉米Δ1-吡咯啉-5-羧酸合成酶基因家族生物信息学分析

Δ1-吡咯啉-5-羧酸合成酶(Delta-1-pyrroline-5-carboxylate synthase,P5CS)是脯氨酸代谢途径的关键酶,对植物抵抗逆境胁迫起至关重要的作用。为了探索P5CS在玉米对逆境胁迫的响应,本文对5个玉米P5CS基因家族蛋白的基本理化性质、二级结构预测、亚细胞定位、基因结构、进化关系及保守基序等方面进行初步生物信息分析。结果显示,5个玉米P5CS蛋白中有3个偏酸性,1个显中性,1个偏碱性;2个以无规则卷曲为主要构成元件,3个P5CS蛋白主要以α白螺旋为主要构成元件;不稳定指数分析发现,4个P5CS蛋白为稳定蛋白;疏水性分析表明,所有的P5CS蛋白为亲水性蛋白;玉米P5CS家族内含子最少含有4个内含子,最多的含有19个内含子;亚细胞定位分析表明,P5CS均蛋白定位于细胞基质;进化分析表明,P5CS家族分3个亚家族。

Bioinformatics Analysis of Delta-1-Pyrroline-5-Carboxylate Synthase Gene Family in Maize

Delta-1-pyrroline-5-carboxylate synthase is a restriction enzyme in proline biosynthesis, and play an important role in responses to biotic and abiotic stresses in plants. In order to study the function of P5CS genes in maize stress response, the physicochemical property, secondary structure, subcellular location, gene structure, evolutionary relationship and conserved motif of maize P5CS genes family were analyzed by the bioinformatics methods. The results showed that there were 3 acidic alkalinity proteins, one neuter protein and one alkalinity protein in the P5CS family. The secondary structure of two of P5CS proteins was random coil, three of them were alpha helix. Instability index analysis found that 4 of the P5CS protein is stabilize protein, hydrophobic analysis showed that all of the P5CS protein were hydrophilic protein, maize P5CS family contains at least four intron, the most one P5CS contains 19 intron, subcellular localization analysis showed that all of P5CS protein localization in cytoplasm, evolution analysis indicated that P5CS family class into three subfamily.