| 蛋白质电泳在杂交水稻种子纯度鉴定中的应用 |
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| 引用本文： | 张凤,李鹏,许鸿川,祁建民,陈伟. 蛋白质电泳在杂交水稻种子纯度鉴定中的应用[J]. 福建农业学报, 2007, 22(3): 235-240 |
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| 作者姓名： | 张凤 李鹏 许鸿川 祁建民 陈伟 |
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| 作者单位： | 1. 福建农林大学生命科学学院,福建,福州,350002;莱芜职业技术学院,山东,莱芜,271100
2. 福建农林大学生命科学学院,福建,福州,350002 |
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| 基金项目： | 福建省重点科技项目(2003N032) |
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| 摘 要： | 以4个三系配套杂交水稻组合及7个杂交稻F1代种子为试验材料,提取其醇溶蛋白和盐溶蛋白,利用SDS-PAGE技术进行图谱分析。结果表明:醇溶蛋白的多态性较弱,差异不大,蛋白质特征带不明显,不适合用于杂交水稻组合和杂交种子的真实性和纯度的检测;而盐溶蛋白的多态性丰富,差异明显,蛋白质特征带稳定,杂交水稻种子蛋白质电泳鉴定与田间小区种植鉴定结果基本吻合。SDS-PAGE技术简便快捷、分离效果好,是一种经济有效的技术,可作为杂交水稻F1代种子纯度蛋白质指纹图谱鉴定。
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| 关 键 词： | 杂交水稻 种子 醇溶蛋白 盐溶蛋白 SDS-PAGE 纯度鉴定 |
| 文章编号： | 1008-0384(2007)03-0235-06 |
| 收稿时间： | 2007-05-08 |
| 修稿时间： | 2007-06-19 |
Application of protein electrophoresis on the seed identification of hybrid rice |
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| ZHANG Feng,LI Peng,XU Hong-chuan,QI Jian-min,CHEN Wei. Application of protein electrophoresis on the seed identification of hybrid rice[J]. Fujian Journal of Agricultural Sciences, 2007, 22(3): 235-240 |
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| Authors: | ZHANG Feng LI Peng XU Hong-chuan QI Jian-min CHEN Wei |
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| Affiliation: | 1. College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China; 2. Laiwu Vocational College Laiwu, Shandong, Laiwu, Shandong 271100, China |
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| Abstract: | Salt-and alcohol-soluble proteins extracted from the seeds of four different hybrid rice and their three lines,as well as seven other F1 hybrid progenies were analyzed by means of SDS-PAGE.The results showed that the polymorphism of alcohol-soluble proteins was not significant enough for authenticity and purity assessment.However,the salt-soluble proteins had a high polymorphism with stable and specific bands.The experiments demonstrated that SDS-PAGE was a simple,rapid,effective and inexpensive method for the identification of hybrid rice seeds. |
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| Keywords: | hybrid rice seeds alcohol-soluble protein saltsoluble protein SDS-PAGE seed identification |
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