| 地被月季组培快繁技术分析及试验研究 |
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| 引用本文： | 杨永花,汉梅兰.地被月季组培快繁技术分析及试验研究[J].甘肃林业科技,2010,35(4):10-12,19. |
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| 作者姓名： | 杨永花 汉梅兰 |
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| 作者单位： | 兰州市园林科学研究所,甘肃兰州730070 |
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| 基金项目： | 兰州市科技局科技公关项目 |
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| 摘 要： | 以MS为基本培养基,附加不同浓度的6-BA、NAA、IBA、KT等植物生长调节剂,对地被月季"猩红梅荻朗"品种的组培快繁技术进行了研究,同时进行了试管苗移栽基质筛选试验。结果表明:适宜的诱导培养基为MS+6-BA1.0mg/L+NAA0.10mg/L,腋芽萌发率达170%,平均芽高为2.6cm;增殖培养基以MS+KT0.5mg/L+NAA0.10mg/L效果最好,增殖系数达7.0,且芽生长健壮;最适宜的生根培养基为1/2MS+IBA0.05mg/L,生根率达96%,且根健壮发达。
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| 关 键 词： | 地被月季 组织培养 MS培养基 快速繁殖 移栽 |
Analysis and Experiment on Rapid Propagation of Groundcover Rose in vitro |
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| YANG Yong-hua,HAN Mei-lan.Analysis and Experiment on Rapid Propagation of Groundcover Rose in vitro[J].Journal of Gansu Forestry Science and Technology,2010,35(4):10-12,19. |
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| Authors: | YANG Yong-hua HAN Mei-lan |
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| Institution: | (Lanzhou Institute of Landscape Science and Technology,Lanzhou Gansu 730010,China) |
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| Abstract: | Based on the basic subculture of MS,with different concentration plant growth regulators of 6-BA,IAA,NAA,and KT,the rapid propagation techniques of groundcover rose in vitro（Scarlet Meidilang） were studied,and the subculture for transplanting of test-tube plantlet was selected.The results showed that the suitable induction culture me-dia was MS＋6-BA 1.0 mg/L ＋NAA 0.10 mg/L,with the sprouting rate of axillary bud up to 170% and average height of bud 2.6 cm.The optimum medium for multiplication was MS＋ KT 0.5mg/L ＋NAA 0.10 mg/L,with the multiplication coefficient up to 7.0,and the buds grewstrongly.The most appropriate medium for rooting was 1/2MS ＋ IBA0.05 mg/L with rooting rate 96%,and root robust and well developed. |
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| Keywords: | Groundcover rose tissue culture MS medium rapid propagation transplanting |
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