烟叶中PPO的抑制效应

比较了硫脲、蔗糖、Ca Cl2 、Co Cl2 、亚硫酸氢钠、柠檬酸、柠檬酸 -氯化钠、柠檬酸 -氯化钠 -亚硫酸氢钠、EDTA、巯基乙醇和 Na Di Ca等 11种 PPO的常用抑制剂对烟叶中 PPO活性的抑制强度。结果表明 ,11种抑制强度由大到小的顺序是 :Na Di Ca、EDTA、巯基乙醇、硫脲、柠檬酸 -氯化钠、柠檬酸 -氯化钠 -亚硫酸氢钠、Ca Cl2 、Co-Cl2 、柠檬酸、亚硫酸氢钠和蔗糖 ;Na Di Ca、EDTA、硫脲和巯基乙醇的抑制效果明显优于其他抑制剂 ,Na Di Ca的抑制效果最佳 ,叶面喷施适宜浓度的抑制剂可有效控制 PPO活性 ,是防止烟叶褐变的有效方法

Effect of different inhibitors on tobacco PPO activity

Inhibition effects between several kinds of PPO inhibitor including thiourea,sucrose,CaCl 2,CoCl 2,citric acid,NaHSO 3,citric acid NaCl,citric acid NaCl NaHSO 3,ethylene diamine tetra acetic acid,mercapto ethanol,and NaDiCa was compared,the experimental result showed that the inhibition power of these inhibitors is not identical,their inhibition power order at same level of concentration stronger toward poorer is:NaDiCa,ethylene diamine tetra acetic acid,mercapto ethanol,thiourea,citric acid NaCl,citric acid NaCl NaHSO 3,CaCl 2,CoCl 2,citric acid,NaHSO 3 and sucrose.The inhibition power of NaDiCa,ethylene diamine tetra acetic acid,mercapto ethanol,and thiourea is much stronger than that of others,and NaDiCa has the strongest inhibition effect.Inhibitor absorbed in tobacco leaves through foliage spray can control activity of PPO effectively,and is an effective way to prevent tobacco leaves from turning brown due to PPO catalysis.