| 利用OneMap软件构建鲤遗传连锁图谱 |
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| 引用本文： | 高国强,常玉梅,匡友谊,梁利群.利用OneMap软件构建鲤遗传连锁图谱[J].水产学报,2010,34(5):649-655. |
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| 作者姓名： | 高国强 常玉梅 匡友谊 梁利群 |
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| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;上海海洋大学水产与生命学院,上海,201306
2. 中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070 |
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| 基金项目： | 国家“九七三”计划资助项目(2004CB117400); 农业部水产生物技术重点开放实验室(2009NYBZS-05); 中央级公益性科研院所基本科研业务费专项资金项目(2009HSYZX-SJ-05) |
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| 摘 要： | 首次使用R环境中的OneMap软件包,以荷包红鲤抗寒品系(♂)和云南大头鲤(♀)为祖父母本所培育的110个F2个体为作图群体,以荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)为主要分子标记,采用远交全同胞家系模型构建鲤的遗传连锁图谱。结果显示,110个F2个体中共产生1513个清晰的fAFLP标记,其中多态性标记911个;另开发多态性的EST标记12个,最后总计923个标记用于构建遗传连锁图谱;采用OneMap软件包构建的遗传图谱含有238个fAFLP标记和8个EST标记分布在50个连锁群上,总图距为2876.64cM,标记间平均间距为14.68cM,图谱覆盖率为66.56%。
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| 关 键 词： | 鲤 遗传连锁图谱 荧光扩增片段长度多态性 OneMap |
| 收稿时间： | 2009/12/15 0:00:00 |
| 修稿时间： | 3/6/2010 12:00:00 AM |
The contruction of genetic linkage map of common carp (Cyprinus carpio L. )using OneMap software |
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| GAO Guo-qiang,CHANG Yu-mei,KUANG You-yi,LIANG Li-qun.The contruction of genetic linkage map of common carp (Cyprinus carpio L. )using OneMap software[J].Journal of Fisheries of China,2010,34(5):649-655. |
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| Authors: | GAO Guo-qiang CHANG Yu-mei KUANG You-yi LIANG Li-qun |
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| Institution: | GAO Guo-qiang1,2,CHANG Yu-mei1,KUANG You-yi,LIANG Li-qun1* (1. Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2. College of Fisheries , Life Sciences,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | The common carp (Cyprinus carpio L. ) is the most extensively cultured fish in the world,as well as in China. Construction of genetic linkage map of common carp is necessary to increase the efficiency of selective breeding,especially complicated traits. So,it is the basis of keeping on the genetic study of a species with construction of full genetic map and location of quantitative trait. In this study,a genetic linkage map has been constructed for the common carp (C. carpio L. ) using the mode of outbreedi... |
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| Keywords: | common carp(Cyprinus carpio) genetic linkage map fluorescent amplification fragment length polymorphism(fAFLP) OneMap |
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