| 立枯丝核菌毒素相对浓度测定方法的研究 |
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| 引用本文： | 马立功,张匀华,季宏平,孟庆林,石凤梅,张威,纪武鹏,王军,刘佳,李易初. 立枯丝核菌毒素相对浓度测定方法的研究[J]. 黑龙江农业科学, 2010, 0(6): 65-67 |
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| 作者姓名： | 马立功 张匀华 季宏平 孟庆林 石凤梅 张威 纪武鹏 王军 刘佳 李易初 |
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| 作者单位： | 黑龙江省农业科学院,植物保护研究所,黑龙江,哈尔滨,150086;黑龙江省农业科学院,植物脱毒苗木研究所,黑龙江,哈尔滨150086;黑龙江省农垦科学院,作物研究所,黑龙江,佳木斯,154007 |
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| 基金项目： | 黑龙江省十五科技攻关重点项目 |
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| 摘 要： | 通过测定立枯丝核菌毒素的紫外吸收光谱,在200~300 nm找出了210、220、241、269和296 nm 5个特征吸收峰。并应用大豆幼根对提取的毒素进行了生物活性的测定。通过对毒素不同浓度与吸光值回归分析,得出了其相对浓度测定的数学计算式。
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| 关 键 词： | 立枯丝核菌毒素 毒素浓度 吸光值 |
Study on Measurement of Comparatively Toxin Concentration of Rhizoctonia solani |
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| MA Li-gong,ZHANG Yun-hua,JI Hong-ping,MENG Qing-lin,SHI Feng-mei,ZHANG Wei,JI Wu-peng,WANG Jun,LIU Jia,LI Yi-chu. Study on Measurement of Comparatively Toxin Concentration of Rhizoctonia solani[J]. Heilongjiang Agricultural Science, 2010, 0(6): 65-67 |
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| Authors: | MA Li-gong ZHANG Yun-hua JI Hong-ping MENG Qing-lin SHI Feng-mei ZHANG Wei JI Wu-peng WANG Jun LIU Jia LI Yi-chu |
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| Affiliation: | 1. Plant Protection Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086 ;2. Plant Virus-free Seedlings Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086 ;3. Crops Institute of Heilongjiang Land Reclamation Academy of Sciences, Jiamusi, Heilongjiang 154007) |
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| Abstract: | The UV absorption spectra of toxin of Rhizoctonia sogani was measured. Five absorption peaks at 210, 220,241, 269 and 296 nm in the UV spectra. And crude toxin to radicle of soybean was biological tested. At last,a direct calculation formula about comparatively toxin concentration was established by regression analysis of different concentrations of toxins and its ultraviolet absorption. |
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| Keywords: | toxin of Rhizoctonia solani toxin concentration absorption value |
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