陈化烟叶产淀粉酶菌株的复筛及产酶条件优化

采用感官评吸的方法,对从陈化烟叶上初筛分离获得的224株产淀粉酶菌株进行复筛,得到3株能显著改善烟叶品质的产淀粉酶菌株A-7、A-8和A-9。对不同浓度酶液改善烟叶品质的分析表明,0.29 U/m L的酶液A-7、80.67 U/m L的A-8、213.33 U/m L的A-9对烟叶评吸指标有所改善。以菌株A-8为进一步研究对象,对其产酶条件进行了优化。结果表明,A-8在37℃、p H 6.0的条件下,震荡培养36 h生物量达到最大,60 h酶活达到最高,发酵条件的优化可使其单位酶活力提高2.7倍。

Screen of Amylase-producing Bacterial Strains of Tabacco and Enzyme Production Conditions Optimize

Based on 224 of amylase-producing bacterial strains isolated from tobacco leaves and their screening for the production of amylase in our previous work,this study aimed to screen amylase-producing bacterial strains which could obviously improve tobacco interior quality by the assessment of sense organ wih smoking,including amylase A-7,A-8,A-9. Different concentrations of the enzyme solution to improve the quality of tobacco leaves shows that 0.29 U/mL of the enzyme solution A-7, 80.67 U/mL of A-8,213.33 U/mL of A-9 on tobacco smoking indicators have improved. The conditon for enzyme production from strain amylase-producing bacterial A-8 is optimized. The result shows that A-8 at pH 6.0,37 ℃conditions,shaking culture 36 hours biomass maximum,60 hours highest activity,the total enzyme activity could be improved by 2.7 times under the optimum conditions.