| 粘质沙雷氏菌中乙偶姻合成途径调控基因的鉴定 |
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| 引用本文： | 张燎原,徐泉明,孙建安,魏东芝,沈亚领.粘质沙雷氏菌中乙偶姻合成途径调控基因的鉴定[J].江西农业大学学报,2012(4):706-711. |
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| 作者姓名： | 张燎原 徐泉明 孙建安 魏东芝 沈亚领 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室;华东理工大学鲁华生物技术研究所生物反应器国家重点实验室 |
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| 基金项目： | 福建省自然科学基金项目(2011J05048);福建省教育厅A类科技项目(JA11089) |
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| 摘 要： | 粘质沙雷氏菌H30中控制乙偶姻合成的调控因子budR被克隆和鉴定。序列分析表明调控因子budR与液化沙雷氏菌MG1中乙偶姻合成调控因子slaR同源性达80%,属lysR型调控因子。调控因子budR突变可导致粘质沙雷氏菌无法合成乙偶姻,引起培养液pH快速下降和菌体生长缓慢,而在培养过程中维持pH7.0,突变菌的生长可获得恢复,暗示budR的功能为调控粘质沙雷氏菌发酵过程中性产物乙偶姻的合成,以阻止发酵液过度酸化。
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| 关 键 词： | 粘质沙雷氏菌 乙偶姻 调控基因 序列分析 功能鉴定 |
Identification of the Regulatory Gene Controlling the Acetoin Fermentation Pathway in Serratia marcescens H30 |
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| ZHANG Liao-yuan,XU Quan-ming,SUN Jian-an,WEI Dong-zhi,SHEN Ya-ling.Identification of the Regulatory Gene Controlling the Acetoin Fermentation Pathway in Serratia marcescens H30[J].Acta Agriculturae Universitis Jiangxiensis,2012(4):706-711. |
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| Authors: | ZHANG Liao-yuan XU Quan-ming SUN Jian-an WEI Dong-zhi SHEN Ya-ling |
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| Institution: | 1.Key Laboratory of Biopesticide and Chemical Biology,Fujian Agriculture and Forestry University,Ministry of Education,Fuzhou 350002,China;2.State Key Laboratory of Bioreactor Engineering,New World Institute of Biotechnology,East China University of Science & Technology,Shanghai 200237,China) |
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| Abstract: | A lysR type regulatory gene,budR involved in acetoin biosynthetic pathway,was cloned and identified from Serratia marcescens H30.Sequence analysis showed that the budR gene shared 80% identities with the slaR gene from S.liquefaciens MG1.Inactivation of the budR gene could result in the absence of acetoin production accompanied with rapid acidification of the broth and entered the stationary phase in advance during the culture.However,when the culture pH was maintained at 7.0,the growth of budR mutant strain could be relieved,suggesting budR gene promoted the neutral compound(acetoin) production to counteract lethal acidification of the broth. |
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| Keywords: | Serratia marcescens acetoin regulatory gene sequence analysis function identification |
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