| 5种方法对蝴蝶干标本DNA的提取效果研究(英文) |
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| 引用本文： | 乔枫,陈振宁,耿贵工,李思未.5种方法对蝴蝶干标本DNA的提取效果研究(英文)[J].Agricultural Science&Technology,2011(8). |
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| 作者姓名： | 乔枫 陈振宁 耿贵工 李思未 |
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| 作者单位： | 青海师范大学青藏高原环境与资源教育部重点实验室;青海农林科学院作物育种栽培研究所; |
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| 基金项目： | Supported by Scientific Research Foundation for Doctors in Qinghai Normal University~~ |
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| 摘 要： | 目的]研究蝴蝶干标本DNA的提取方法。方法]采用5种不同的方法(SDS法、SDS-巯基乙醇-酚法、CTAB法、饱和NaCl法、SDS-巯基乙醇-氯仿法)对蝴蝶干标本DNA进行提取。结果]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法适合蝴蝶干标本基因组DNA的提取,并且能成功地应用于蝴蝶线粒体COI、EF-1α和CytB基因的PCR扩增。结论]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法可以用于研究蝴蝶干标本DNA的抽提,为蝴蝶的分子系统进化研究奠定了基础。
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| 关 键 词： | 蝴蝶 干标本 DNA提取 |
Study on Five Methods for Extracting DNA from Dried Butterfly Specimen |
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| QIAO Feng,CHEN Zhen-ning,GENG Gui-gong,LI Si-wei.Study on Five Methods for Extracting DNA from Dried Butterfly Specimen[J].农业科学与技术(英文版),2011(8). |
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| Authors: | QIAO Feng CHEN Zhen-ning GENG Gui-gong LI Si-wei |
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| Institution: | QIAO Feng1,CHEN Zhen-ning1,GENG Gui-gong2,LI Si-wei11.Key Lab of Environment and Resource in Qinghai-Tibet Platean of Ministry of Education,Qinghai Normal University,Xining 810008,2.Institute of Crop Breeding and Cultivation,Qinghai Academy of Agriculture and Forestry,Xining 810016 |
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| Abstract: | Objective]The aim was to find out the optimal methods for extracting DNA from dried butterfly specimen.Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl method,SDS-mercaptoethanol-chloroform methods were employed to extract DNA from dried butterfly specimen.Result]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method were suitable for genomic DNA extraction from dried butterfly samples,and the DNA e... |
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| Keywords: | Butterfly Dried specimens DNA extraction |
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