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| 磷胁迫下油菜幼苗叶片差异蛋白表达的初步分析 | |
| 引用本文： | 余永芳,赵丹丹,李玉琴,牛银银,田保明,臧新. 磷胁迫下油菜幼苗叶片差异蛋白表达的初步分析[J]. 河南农业科学, 2011, 40(8): 89-91,112 |
| 作者姓名： | 余永芳 赵丹丹 李玉琴 牛银银 田保明 臧新 |
| 作者单位： | 郑州大学生物工程系,河南郑州,450001 |
| 摘 要： | 以油菜品种中油821为材料,利用双向电泳技术研究足磷和缺磷条件下油菜幼苗叶片差异表达的蛋白质,进而为揭示油菜耐低磷胁迫的分子机制奠定基础.结果表明:经电泳、PDQuest分析后,缺磷处理下差异显著的蛋白质点有38个,19个表达量显著上调,19个显著下调.磷胁迫下蛋白表达量的差异说明逆境下植物体可通过多种蛋白的协调作用来... |
| 关 键 词： | 油莱 磷胁迫 双向电泳 蛋白质组学 差异蛋白表达 |
Preliminary Analysis of Differential Proteins of Brassica napus Leaves under Phosphorous Deficiency |
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| YU Yong-fang,ZHAO Dan-dan,LI Yu-qin,NIU Yin-yin,TIAN Bao-ming,ZANG Xin. Preliminary Analysis of Differential Proteins of Brassica napus Leaves under Phosphorous Deficiency[J]. Journal of Henan Agricultural Sciences, 2011, 40(8): 89-91,112 | |
| Authors: | YU Yong-fang ZHAO Dan-dan LI Yu-qin NIU Yin-yin TIAN Bao-ming ZANG Xin |
| Affiliation: | YU Yong-fang,ZHAO Dan-dan,LI Yu-qin,NIU Yin-yin,TIAN Bao-ming,ZANG Xin(Bioengineering Department,Zhengzhou University,Zhengzhou 450001,China) |
| Abstract: | The proteome changes in the seedling leaves of Zhongyou 821 was investigated by 2-DE,which could lay the foundation for the molecule mechanism of the rapeseed endured low phosphor.The results showed that,analysed by 2-DE,PDQuest software,the remarkable difference points of the young plants treated with no enough phosphoric were 38,and 19 proteins were up-regulated,and the other 19 points were down-regulated,which revealed that many proteins invovled in the physiological process under phosphorous deficency. |
| Keywords: | Rapeseed Phosphorus deficiency Two-dimensional gel electrophoresis Proteomics Differential protein expression |
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