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| 芝麻NBS - LRR类抗病基因同源序列的分离与分析 | |
| 引用本文： | 高树广,刘红彦,王俊美,倪云霞,田保明.芝麻NBS - LRR类抗病基因同源序列的分离与分析[J].河南农业科学,2011,40(9):81-85. |
| 作者姓名： | 高树广 刘红彦 王俊美 倪云霞 田保明 |
| 作者单位： | 1. 郑州大学生物工程系,河南郑州450001;河南省农业科学院植物保护研究所,河南郑州450002 2. 河南省农业科学院植物保护研究所,河南郑州450002;河南省农作物病虫害防治重点实验室,河南郑州450002 3. 郑州大学生物工程系,河南郑州,450001 |
| 基金项目： | 国家芝麻产业技术体系(nycytx-20-1-05) |
| 摘 要： | 分离和分析芝麻抗、感茎点枯病材料的抗病基因同源序列(resistance gene analogs,RGAs),可以为芝麻抗茎点枯病相关基因的克隆奠定基础.根据已知植物抗病基因的NBS - LRR类保守结构域,合成了1对简并引物和2对特异引物,对6个抗茎点枯病芝麻品种中的抗病基因进行扩增,结果得到11条抗病基因同源序列... |
| 关 键 词： | 芝麻 茎点枯病 NBS -LRR 抗病基因同源序列 |
Isolation and Analysis of NBS-LRR Resistance Gene Analogs from Sesame |
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| GAO Shu-guang,LIU Hong-yan,WANG Jun-mei,NI Yun-xia,TIAN Bao-ming.Isolation and Analysis of NBS-LRR Resistance Gene Analogs from Sesame[J].Journal of Henan Agricultural Sciences,2011,40(9):81-85. | |
| Authors: | GAO Shu-guang LIU Hong-yan WANG Jun-mei NI Yun-xia TIAN Bao-ming |
| Institution: | GAO Shu-guang1,2,LIU Hong-yan2,3,WANG Jun-mei2,NI Yun-xia2,TIAN Bao-ming1(1.Bioengineering Department,Zhengzhou University,Zhengzhou 450001,China,2.Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,3.Henan Key Laboratory of Crop Pest Control,China) |
| Abstract: | Cloning and analysis of resistance gene analogs(RGAs)of resistant/susceptible sesame to Macrophomina phaseoli could lay the foundation for cloning resistance-related genes.One pair of degenerate primers and two pairs of specific primers were designed based on nucleotide binding site(NBS)-leucine rich repeats(LRR) conserved domain of reported resistance genes from other plants to amplify resistance genes from six sesame varieties resistant to Macrphomina phaseoli and eleven RGAs were obtained.BLAST X analysi... |
| Keywords: | Sesame Stem blight NBS-LRR Resistance gene analogs |
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