| 金标记免疫共振散射光谱法测定牛初乳中免疫球蛋白G |
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| 引用本文： | 刘梯楼,孙双姣. 金标记免疫共振散射光谱法测定牛初乳中免疫球蛋白G[J]. 湖南农业科学, 2011, 0(17) |
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| 作者姓名： | 刘梯楼 孙双姣 |
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| 作者单位： | 邵阳医学高等专科学校,湖南邵阳,422000 |
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| 基金项目： | 湖南省自然科学基金项目(10c0298) |
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| 摘 要： | 采用柠檬酸三钠还原法制备了粒径为9 nm的金纳米微粒。结果表明:在pH 7.0的Tris-HCl缓冲溶液中,金标记羊抗牛免疫球蛋白G(IgG)与牛IgG产生特异性结合,羊抗牛IgG包裹的金纳米微粒释放出来,在聚乙二醇-20000的作用下发生聚集,导致体系在580 nm处的共振散射峰增强。当牛IgG浓度在0.013 3～1.33μg/mL范围内,随着牛IgG浓度的增加,共振散射强度线性增强,方法的检出限为0.007 95μg/mL,用于定量分析牛初乳中的IgG,结果令人满意。
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| 关 键 词： | 牛IgG 胶体金标记羊抗牛IgG 免疫反应 共振散射 |
Determination of IgG in Bovine Colostrum by Using Colloid Gold Labeling Immune Resonance Scattering Spectral Assay |
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| LIU Ti-lou,SUN Shuang-jiao. Determination of IgG in Bovine Colostrum by Using Colloid Gold Labeling Immune Resonance Scattering Spectral Assay[J]. Hunan Agricultural Sciences, 2011, 0(17) |
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| Authors: | LIU Ti-lou SUN Shuang-jiao |
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| Affiliation: | LIU Ti-lou,SUN Shuang-jiao(Shaoyang Medical College,Hunan 422000,PRC) |
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| Abstract: | 9.0 nm of gold nano-particles were prepared by using the method of trisodium citrate reduction method.The results showed that in the pH 7.0 of Tris-HCl buffer solution,the colloid gold labeling goat anti-bovine IgG was specific binding with the bovine IgG,the gold nano-particles were released from the goat anti-bovine IgG,and then aggregated to bigger size particles with the action of polyethylene glycol-2000(PEG-2000),which leaded the intensity of resonance scattering peaks at 580 nm to be enhanced greatly... |
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| Keywords: | bovine IgG colloid gold labeling goat anti-bovine IgG immunoreaction resonance scattering |
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