| 猪胸膜肺炎放线杆菌16SrDNA基因的克隆及序列分析 |
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| 引用本文： | 沈萍,王喜军.猪胸膜肺炎放线杆菌16SrDNA基因的克隆及序列分析[J].湖南农业科学,2011(15):154-156,161. |
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| 作者姓名： | 沈萍 王喜军 |
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| 作者单位： | 1. 湖南生物机电职业技术学院动科系,湖南长沙,410127
2. 长沙市动物防疫监督站,湖南长沙,410012 |
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| 摘 要： | 为了阐明猪胸膜肺炎放线杆菌16S rDNA基因的遗传变异情况,选择猪胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)的16S rDNA基因设计引物,应用聚合酶链反应(PCR)扩增APP的16S rDNA基因的部分序列,将该产物克隆到pMDI8-T载体上,重组质粒通过菌落PCR鉴...
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| 关 键 词： | 猪胸膜肺炎放线杆菌 16S rDNA基因 克隆及序列分析 |
Cloning and Sequence Analysis of 16S rDNA Gene of Actinobacillus pleuropneumoniae |
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| SHEN Ping,WANG Xi-jun.Cloning and Sequence Analysis of 16S rDNA Gene of Actinobacillus pleuropneumoniae[J].Hunan Agricultural Sciences,2011(15):154-156,161. |
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| Authors: | SHEN Ping WANG Xi-jun |
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| Institution: | SHEN Ping1,WANG Xi-jun2(1.Department of Animal Science,Hunan Biological & Electromechanical Polytechnic,Changsha 410127,PRC,2.Changsha Animal Epidemic Prevention and Supervise Station,Changsha 410012,PRC) |
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| Abstract: | This study aimed to clarify the genetic variation situation of 16S rDNA of Actinobacillus pleuropneumoniae(APP).The 16S rDNA gene of APP was selected to design primers,the partial sequences of 16S rDNA gene of APP were amplified by using polymerase chain reaction(PCR),and the obtained products were cloned into pMD18-T vector.After the recombinant plasmids were identified by colony PCR,the positive colonies were sequenced and analyzed.The results showed that the homology between obtained Changsha strain(CS) ... |
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| Keywords: | Actinobacillus pleuropneumoniae(APP) 16S rDNA gene cloning and sequence analysis |
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