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| 拟单性木兰种子DNA提取方法的研究 | |
| 引用本文： | 杨萍,胡永萍,王正加.拟单性木兰种子DNA提取方法的研究[J].浙江林业科技,2004,24(1):24-26,38. |
| 作者姓名： | 杨萍 胡永萍 王正加 |
| 作者单位： | 1. 浙江林学院,生命科学学院,浙江,临安,311300;浙江工业大学,生物与环境学院,浙江,杭州,310014 2. 浙江省杭州天目药业研发中心,浙江,临安,311300 3. 浙江林学院,生命科学学院,浙江,临安,311300 |
| 摘 要： | 以云南拟单性木兰种子为材料,对三种DNA的提取方法(CTAB法、SDS法、高盐-低pH值法)进行了比较.结果表明:CTAB法最适于拟单性木兰种子DNA的提取,且适宜于RAPD分析,SDS法次之,高盐-低pH值法不适合. |
| 关 键 词： | 拟单性木兰种子 DNA提取 |
| 文章编号： | 1001-3776(2004)01-0024-03 |
Study on Separation Method of DNA from Parakmeria Seed |
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| YANG Ping,HU Yong-ping,WANG Zheng-jia.Study on Separation Method of DNA from Parakmeria Seed[J].Journal of Zhejiang Forestry Science and Technology,2004,24(1):24-26,38. | |
| Authors: | YANG Ping HU Yong-ping WANG Zheng-jia |
| Institution: | YANG Ping1,2,HU Yong-ping3,WANG Zheng-jia1 |
| Abstract: | Comparison test was carried out on 3 separation methods of DNA from Parakmeria yunnanensis seed resulted that CTAB method was the best one, then SDS, and high salinity-low pH was not recommended. The methods could be applied in identification for seed purity of Parakmeria seed. |
| Keywords: | RAPD |
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