雨生红球藻规模化培养工艺的构建与应用

针对雨生红球藻规模化培养中的工艺流程、生物污染、温度控制、溶氧解析和pH值稳定等问题,于2003年到2005年,在山东省潍坊地区,开展了雨生红球藻中试规模的论证研究。建立了可用于雨生红球藻规模化生产的3种不同类型的光生物反应器(柱式、管道、生物幕)和人工调节太阳能的温控车间,并构建了一套二步规模化培养工艺流程。结果表明,研究构建的培养模式降低了培养过程中的潜在风险,并大大提高了雨生红球藻虾青素含量。与传统培养方式相比,年培养天数大约提高了3倍。在每个批次培养中,细胞生长培养效率提高了至少1.75倍,虾青素累积培养所需时间缩短了40%,虾青素含量提高了4倍。总体来看,研究构建的新工艺比传统开放模式的生产效率有很大提高,全年共计提高了35倍。

Technological assembly and its application in a pilot scale culture of haematococcus pluvialis

Some key problems existed in microalgal application such as technological assembly, bio-contamination protection, temperature controlling, dissolved oxygen discharging, pH stabilization, etc were conducted in a pilot scale culture of haematococcus pluvialis in Weifang from 2003 to 2005. Three new enlarged photobioreactors (column, tubular and biofilm), an effective and economic temperature controlled plant as well as two-step culture mode was established for microalgal application. Data also showed that the risk of bio-contamination usually blocking the scale-up process of haematococcus pluvialis culture was well reduced, meanwhile astaxanthin content was greatly enriched. Compared with the traditional technic, some crucial parameters in the new culture mode were greatly improved as below: 3 folds prolong of the cultivation period in a year, 40% decrease of the astaxanthin accumulation period, 1.75 folds increase in effeciency at least and 4 folds increase in astaxanthin content in the biomass. Therefore, the astaxanthin production efficiency in haematococcus pluvialis was about 35 folds higher than that of traditional technique.