| 番茄基因转化中抗生素筛选压力的确定方法 |
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| 引用本文： | 张寅玲,刘艳军,张伟玉,杨静慧,柳树梧.番茄基因转化中抗生素筛选压力的确定方法[J].西南园艺,2005,33(5):4-6. |
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| 作者姓名： | 张寅玲 刘艳军 张伟玉 杨静慧 柳树梧 |
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| 作者单位： | 1. 天津医药教育中心,300130
2. 天津农学院,300384 |
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| 基金项目： | 天津市自然科学基金资助（023614211）,天津市生物技术中心资助,天津市教委资助（2001）,天津农学院青年重点基金资助. |
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| 摘 要： | 在以抗生素为筛选标记的基因转化中。确定适宜的筛选压力是提高基因转化率的前提。本研究以卡那霉素为例，通过在10mg／L，20mg／L，30mg／L，50mg／L，100mg／L的筛选压力下，转化和非转化番茄子叶外植体的生长表现、愈伤组织和芽的分化率．来确定适宜的筛选浓度。本实验以该方法找到的适宜的卡那霉素筛选浓度为50mg／L。以此浓度为筛选压力进行遗传转化所获得的部分再生苗经southern blot鉴定．均为转化株。
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| 关 键 词： | 基因转化 番茄 抗生素 卡那霉素 筛选压力 |
| 文章编号： | 1008-1488（2005）05-004-03 |
| 收稿时间： | 2005-06-10 |
| 修稿时间： | 2005年6月10日 |
The Method to Confirm the Selective Concentration of Antibiotic in Genetic Transformation |
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| Zhang Yinling,LIU Yanjun,Zhang Weiyu,Yang Jinghui,Liu Shuwu.The Method to Confirm the Selective Concentration of Antibiotic in Genetic Transformation[J].Southwest Horticulture,2005,33(5):4-6. |
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| Authors: | Zhang Yinling LIU Yanjun Zhang Weiyu Yang Jinghui Liu Shuwu |
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| Abstract: | In order to confirm the optimum selective concentration of antibiotic in genetic transformation, kanamycin was selected as a case in this paper. It had been found that the optimum selective concentration of kanamycin was 50 mg/L by the investigation of the effects upon the growth and differentiation of explants of tomato cotyledon on the media containing different concentrations that were 10 mg/L,20 mg/L,30 mg/L,50 mg/L,100 mg/L, respectively. Some of shoots of transformation that selected by 50 g/L of kanamycin were confirmed by Southern blot. |
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| Keywords: | Genetic Transformation Tomato Antibiotic Kanamycin |
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