Influence of water potential on nitrification and structure of nitrifying bacterial communities in semiarid soils |
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| Authors: | Deirdre B Gleeson Anke M Herrmann Stephen J Livesley Daniel V Murphy |
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| Institution: | aSoil Biology Group, School of Earth and Geographical Sciences M087, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia;bInstitute for Research on Environment and Sustainability, Newcastle University, NE1 7RU, United Kingdom;cSchool of Forest and Ecosystem Science, The University of Melbourne, 500 Yarra Boulevard, Richmond, Vic 3121, Australia |
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| Abstract: | The objective of this study was to examine the relationship between soil water potential, nitrifier community structure and nitrification activity in semiarid soils. Soils were collected after a 5-month dry period (end of summer) and subsequently rewetted to specific water potentials and incubated for 7 days prior to analysis of nitrification activity and nitrifier community structure. The approach used in this study targeted a 491bp segment of the amoA gene which encodes the active site of the ammonia monooxygenase enzyme, which is the key enzyme for all aerobic ammonia oxidisers. amoA serves as a useful target for environmental studies since it is both specific and universal for all ammonia oxidisers and reflects the phylogeny of the ammonia oxidisers. Our results suggest that in semiarid soils water potential plays a key role in determining the structure of ammonia oxidising bacteria (AOB), and that additionally AOB community structure is correlated to potential nitrification rate in these soils. |
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| Keywords: | Ammonia oxidising bacteria Nitrification Rewetting stress Semiarid soils |
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