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| 提高水稻花培育种效率研究 | |
| 引用本文： | 苗立新,李鑫,谢丽霞,佟彤,何功志,王力嘉.提高水稻花培育种效率研究[J].北方水稻,2010,40(1):15-17,37. |
| 作者姓名： | 苗立新 李鑫 谢丽霞 佟彤 何功志 王力嘉 |
| 作者单位： | 1. 辽宁省盐碱地利用研究所,辽宁,盘锦,124010 2. 盘锦东禾种业有限公司,辽宁,盘锦,124010 3. 大石桥市茂洋种子有限公司,辽宁,营口,115000 |
| 摘 要： | 通过对7个杂交组合F1代的花药培养,探讨了花培材料不同低温处理、不同诱导分化培养基激素配比、不同添加物以及培养条件对花药培养效率的影响,结果表明:低温预处理可提高花培的诱愈率,以8℃、8 d处理效果最好;改良N6培养基+YD2处理为粳稻、偏粳材料愈伤组织诱导最佳培养基,MS培养基+FH4处理为粳稻、偏粳材料愈伤组织分化的最佳培养基;多效唑(MET)对绿苗分化具有很大的促进作用;水解乳蛋白(LH)等有机附加物对多数材料的愈伤组织诱导和分化都有一定的作用;光、温培养条件对愈伤组织诱导分化有影响。 |
| 关 键 词： | 花培 愈伤组织诱导率 绿苗分化率 育种效率 |
Study on Increasing Pollen Culture Breeding Efficiency in Rice |
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| MIAO Li-xin,LI Xin,XIE Li-xia,TONG Tong,HE Gong-zhi,WANG Li-jia.Study on Increasing Pollen Culture Breeding Efficiency in Rice[J].Reclaiming and Rice Cultivation,2010,40(1):15-17,37. | |
| Authors: | MIAO Li-xin LI Xin XIE Li-xia TONG Tong HE Gong-zhi WANG Li-jia |
| Institution: | 1 Liaoning Saline or Alkaline Land Utilization and Research Institute/a>;Panjin Liaoning 124010/a>;China/a>;2 Panjin Donghe Seed Co./a>;Ltd./a>;3 Dashiqiao Maoyang Seed Co.Ltd./a>;Yingkou Liaoning 115000/a>;China |
| Abstract: | The effect of pollen culture conditions on pollen culturing efficiency of rice was studied in the conditions of pre-low temperature treatment,different induction hormone formula,and additives with F1 of seven crosses as entries.The result showed that pre-low temperature,8 ℃ for 8 d,was able to increase callus induction rate;modified N6+YD2 medium was a suitable callus induction medium for japonica or partial japonica rice materials and MS+FH4 medium was favorable for callus differentiation for japonica or partial japonica rice materials;MET promoted green seedling differentiation and lactoalbumin hydrolysate(LH) had effect on callus induction of most materials;light and temperature also affected callus induction and differentiation. |
| Keywords: | Pollen culture Callus tissue induction rate Green seedling differentiation rate Breeding efficiency |
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