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| 基于高分辨率熔解曲线技术快速筛选黄瓜SNP1 | |
| 引用本文： | 兰青阔,张桂华,王永,赵新,朱珠,孙玉河,郭永泽,程奕. 基于高分辨率熔解曲线技术快速筛选黄瓜SNP1[J]. 分子植物育种, 2011, 0(5): 642-647 |
| 作者姓名： | 兰青阔 张桂华 王永 赵新 朱珠 孙玉河 郭永泽 程奕 |
| 作者单位： | 天津市农业科学院中心实验室;天津科润农业科技股份有限公司黄瓜研究所 |
| 基金项目： | 天津市科技支撑项目(10ZCGYNC00400);天津市应用基础及前沿技术研究计划(10JCYBJC09300)共同资助 |
| 摘 要： | 为筛选用以黄瓜纯度检验和品种鉴定的SNP位点,以17个黄瓜杂交品种及其34份亲本为SNP筛选群体,利用高分辨率熔解曲线(high resolution melting,HRM)技术筛选SNP位点.本研究通过HRM分析和焦磷酸测序确认,获得CLA0(TT/--)、CLA1(C/T)和CLA6(A/G)三个SNP位点,群体... |
| 关 键 词： | 黄瓜 SNP标记 高分辨率熔解曲线 |
Discovery of Single Nucleotide Polymorphism in Cucumber Based on High Resolution Melting Analysis |
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| Lan Qingkuo,Zhang Guihua,Wang Yong, Zhao Xin,Zhu zhu,Sun Yuhe,Guo Yongze,Cheng Yi. Discovery of Single Nucleotide Polymorphism in Cucumber Based on High Resolution Melting Analysis[J]. Molecular Plant Breeding, 2011, 0(5): 642-647 | |
| Authors: | Lan Qingkuo Zhang Guihua Wang Yong Zhao Xin Zhu zhu Sun Yuhe Guo Yongze Cheng Yi |
| Affiliation: | 1 Central Lab of Tianjin Agriculture Academy Sciences, Tianjin, 300381; 2 Tianjin Kernel Cucumber Research Institute of Tianjin Agriculture Academy of Science, Tianjin, 300192 |
| Abstract: | In order to explore SNP sites for cucumber purity test and cultivar identification, high resolution melting (HRM) was used to screening SNP site from the group including 17 cucumber hybrid and their parents lines. By HRM analysis and Pyrosequencing confirmation, we got three SNP sites, named CLA0(TT /--), CLA1(C/T) and CLA6(A/G), whose polymorphism information (PIC) were 0.549, 0.366 and 0.477 respectively. And the three SNP sites are suited to purity test for 91.7% of the 12 commercial cucumber hybrid cultivars and have the potentiality for cultivar identification. |
| Keywords: | Cucumber Single nucleotide polymorphism (SNP) High resolution melting (HRM) |
| 本文献已被 CNKI 等数据库收录！ | |