不同连作年限菊花根际土壤微生物多样性变化特征

为揭示连作菊花根际土壤微生物群落结构和多样性变化特征,以不同连作年限菊花根际土壤为试验材料,采用高通量测序技术(Illumina-MiSeq)和生物信息学方法,分析无连作(CK)、连作2年(2a)、连作9年(9a)菊花根际土壤细菌和真菌的丰富度、多样性指数和群落结构,以期为菊花连作障碍防控提供理论支撑。结果表明,2a、9a根际土壤细菌数量和多样性较CK显著(P<0.05)增加,2a和9a的群落结构相似,共有OTU(operational taxonomic unit)比例高。连作对菊花根际土壤真菌群落多样性和丰度影响较小,但对真菌群落组成影响显著,2a、9a根际土壤真菌接合菌门、担子菌门和被孢霉属等群落丰度减少,明梭孢属、镰刀菌属、土赤壳菌等群落丰度增加。菊花连作会显著增加其根际土壤细菌数量和多样性,显著影响真菌群落结构组成。

Variation characteristics of soil microbial diversity in chrysanthemum rhizosphere soil with different continuous cropping years

In order to reveal the community structure and diversity characteristics of microbe in chrysanthemum rhizosphere soil under continuous cropping, taking the rhizosphere soil of chrysanthemum with different continuous cropping years as the research object, high-throughput sequencing technology (Illumina-MiSeq) and bioinformatics were used to analyze the bacterial and fungal community composition and diversity in chrysanthemum rhizosphere soil of no continuous cropping (CK), continuous cropping for 2-years (2a) and continuous cropping for 9 years (9a). The results showed that the number and diversity of bacteria in rhizosphere soil of chrysanthemum in 2a and 9a treatments were significantly (P<0.05) higher than those of CK. Continuous cropping had little influence on the fungal community diversity and abundance, but had significant influence on the fungal community composition. Zygomycota, Basidiomycota, Mortierella and other microbial communities were decreased, and the abundance of microbial communities such as Monographella, Fusarium, Ilyonectria and other microbial community were increased in 2a and 9a treatment, compared with CK. Continuous cropping of chrysanthemum significantly increased the number and diversity of bacteria, and significantly affected the composition of fungal community in the rhizosphere soil.