Organization and promoter analysis of a tiger shrimp Penaeus monodon single WAP domain-containing protein gene |
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Authors: | Jyh Yih Chen Chen Jian Chyi Lin Sheng Hwa Pan Chia Yu Kuo Ching Ming |
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Institution: | (1) Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Road, Jiaushi, 262 Ilan, Taiwan;(2) Department of Biotechnology, Southern Taiwan University of Technology, 1 Nan-Tai Street, Yung-Kang City, 710 Tainan, Taiwan |
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Abstract: | The main function of the single whey acidic protein domain (SWD)-containing protein in shrimp is unknown. To elucidate the
function of the SWD-containing protein in vivo, the SWD-containing protein gene was isolated and characterized. A 9.3-kb shrimp SWD-containing protein gene, and a 3.9-kb
3′-flanking region. The shrimp SWD-containing protein gene contained three exons and two introns. Different fragments of the
shrimp SWD-containing protein 5′-flanking region were transfected into HeLa cells. The promoter activities were assayed by
basal human chorionic gonadotropin (HCG), luteinizing hormone-releasing hormone (LRH), and gonadotropin-releasing hormone
(GnRH) treatments. The in vitro actions of the SWD-containing protein promoter expression pattern were studied by transfection of an SWD-containing protein
promoter (1 kb)-driven green fluorescent protein (GFP) encoding the GFP cDNA transgene into the HeLa cell line, which was
then microinjected into zebrafish Danio rerio embryos. These results indicate that the shrimp SWD-containing protein promoter might play an important role in gene regulation
of sex hormones in mammalian cell lines and in gene regulation of developmental stages in zebrafish. |
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Keywords: | promoter analysis single WAP domain-containing protein gene tiger shrimp |
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