| 生长温度对烤烟生物碱含量及烟碱代谢相关酶基因表达的影响 |
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| 引用本文： | 金云峰,王莎莎,张建波,杨慧芹,逄涛,李军营,龚明. 生长温度对烤烟生物碱含量及烟碱代谢相关酶基因表达的影响[J]. 热带作物学报, 2016, 37(3): 555-567. DOI: 10.3969/j.issn.1000-2561.2016.03.020 |
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| 作者姓名： | 金云峰 王莎莎 张建波 杨慧芹 逄涛 李军营 龚明 |
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| 作者单位： | 1. 云南师范大学生命科学学院 生物能源持续开发利用教育部工程研究中心 云南省生物质能与环境生物技术重点实验室 云南昆明 650500;2. 云南省烟草农业科学研究院,云南昆明,650031 |
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| 基金项目： | 国家烟草专卖局科技专项“清香型特色优质烟叶开发”[No. 110201101003（TS-03）]；云南省烟草公司科技项目（No. 2014YN16）。 |
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| 摘 要： | ![]()
以云烟87为材料,采用人工气候室模拟云南江川、贵州遵义和河南襄县的气温条件,分别在烟草移栽-团棵、团棵-现蕾及成熟期进行不同温度处理,研究不同生长温度对烟碱、新烟碱、降烟碱和假木贼碱含量以及烟碱代谢关键酶基因表达的影响。结果表明:在3个不同生育期中,较低的生长温度均促进了烟碱的生物合成及其在根系中的积累。在移栽-团棵期,低温处理下根系中的烟碱含量升高至0 d时的1.54倍,而同时期叶片中的烟碱含量显著低于根系;进入团棵-现蕾期以及成熟期后,较高的生长温度则更有利于叶片中烟碱的积累,其烟碱含量较0 d时高约1.80~4.38倍;新烟碱、降烟碱和假木贼碱的含量变化趋势与烟碱基本相似。总体而言,在不同生育期,烟碱的合成和器官水平上的分布不同程度地受到生长温度的影响,成熟期温度对烟碱量的影响最大,具有相对较高生长温度条件的河南襄县更有利于叶片中烟碱的积累。本研究结果揭示了温度调节烟草生物碱含量及烟碱代谢的作用基础,在一定程度上解释了3个地区烟叶香型风格存在差异的原因。
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| 关 键 词： | 烟草 生长温度 生物碱含量 烟碱代谢 相关基因表达 |
Influence of Growth Temperature on Alkaloids Contents and Expression of Key Genes Related to Nicotine Metabolism in Nicotiana tabacum L. |
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| JIN Yunfeng,WANG Shash,ZHANG Jianbo,YANG Huiqin,PANG Tao,LI Junying and GONG Ming. Influence of Growth Temperature on Alkaloids Contents and Expression of Key Genes Related to Nicotine Metabolism in Nicotiana tabacum L.[J]. Chinese Journal of Tropical Crops, 2016, 37(3): 555-567. DOI: 10.3969/j.issn.1000-2561.2016.03.020 |
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| Authors: | JIN Yunfeng WANG Shash ZHANG Jianbo YANG Huiqin PANG Tao LI Junying GONG Ming |
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| Affiliation: | School of Life Sciences, Yunnan Normal University / Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education / Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province;School of Life Sciences, Yunnan Normal University / Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education / Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province;School of Life Sciences, Yunnan Normal University / Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education / Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province;School of Life Sciences, Yunnan Normal University / Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education / Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province;Yunnan Academy of Tobacco Agricultural Sciences;Yunnan Academy of Tobacco Agricultural Sciences;School of Life Sciences, Yunnan Normal University / Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education / Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province |
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| Abstract: | ![]()
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| Keywords: | Tobacco Growth temperature Alkaloid content Nicotine metabolism Gene expression profiles |
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