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| 天门冬组织培养条件的优化 | |
| 引用本文： | 潘丽梅,马小军,韦树根,王晓峰,柯芳. 天门冬组织培养条件的优化[J]. 湖北农业科学, 2012, 51(19): 4396-4398 |
| 作者姓名： | 潘丽梅 马小军 韦树根 王晓峰 柯芳 |
| 作者单位： | 1. 中国医学科学院,北京协和医学院药用植物研究所广西分所/广西药用资源保护与遗传改良重点实验室,南宁530023 2. 中国医学科学院,北京协和医学院药用植物研究所广西分所/广西药用资源保护与遗传改良重点实验室,南宁530023;中国医学科学院,北京协和医学院药用植物研究所,北京 100193 |
| 基金项目： | 广西科学研究与技术开发计划项目(桂科攻0815005-2) |
| 摘 要： | 以天门冬(Asparagus cochinchinensis)幼嫩茎段为外植体,考察1 g/L HgCl2灭菌时间对外植体的消毒效果,以及激素浓度组合对外植体愈伤组织、不定芽的诱导和不定芽生根的影响.结果表明,1 g/L HgCl2处理7 min,外植体褐化率和污染率较低；MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.2 mg/L KT培养基中的愈伤组织诱导率最高,且生长势最强,MS+1.0 mg/L 6-BA+0.2 mg/L NAA+0.2 mg/L KT培养基中的出芽率最高；1/2MS+0.5 mg/L NAA+0.5 mg/L IBA培养基中生根率最高,可达76％. |
| 关 键 词： | 天门冬(Asparagus cochinchinensis) 茎段 组织培养 |
Optimization of Tissue Culture Conditions for Asparagus cochinchinensis |
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| PAN Li-mei , MA Xiao-jun , WEI Shu-gen , WANG Xiao-feng , KE Fang. Optimization of Tissue Culture Conditions for Asparagus cochinchinensis[J]. Hubei Agricultural Sciences, 2012, 51(19): 4396-4398 | |
| Authors: | PAN Li-mei MA Xiao-jun WEI Shu-gen WANG Xiao-feng KE Fang |
| Affiliation: | a(a.Guangxi Branch,Institute of Medicinal Plant Development,Peking Union Medical College/Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Nanning 530023,China;b.Institute of Medicinal Plant Development,Peking Union Medical College,Beijing 100193,China) |
| Abstract: | The young stems of Asparagus cochinchinensis(Lour.)Merr.were used as explants to study the disinfection effect of 1 g/L HgCl2 with different treatment times,and the effect of different hormone combinations on induction rate of callus,adventitious buds and rooting.The results showed that the browning and infection rates of explants were low when sterilized for 7 min by 1 g/L HgCl2.The optimum medium for callus induction was MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.2 mg/L KT.The optimized medium for adventitious buds induction was MS+1.0 mg/L 6-BA+0.2 mg/L NAA+0.2 mg/L KT.The medium with the highest rooting rate(76%) was 1/2MS+0.5 mg/L NAA+0.5 mg/L IBA. |
| Keywords: | Asparagus cochinchinensis(Lour.)Merr. stem segment tissue culture |
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