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| 广西巴马小型猪氟烷基因PCR-RFLP分析 | |
| 引用本文： | 严雪瑜,黄云,严小东,蒋钦杨,郭亚芬,蒋和生. 广西巴马小型猪氟烷基因PCR-RFLP分析[J]. 湖北农业科学, 2012, 51(18): 4144-4146 |
| 作者姓名： | 严雪瑜 黄云 严小东 蒋钦杨 郭亚芬 蒋和生 |
| 作者单位： | 1. 广西大学动物科学技术学院,南宁,530004 2. 广西防城港市动物疫病预防与控制中心,广西防城港,538021 |
| 基金项目： | 广西科学研究与技术开发计划项目(桂科攻10100005-11) |
| 摘 要： | 采用PCR-RFLP分析了广西巴马小型猪的氟烷基因类型.结果显示,PCR扩增获得广西巴马小型猪氟烷基因片段长452 bp,其碱基序列与GenBank公布的普通猪同源性为99.6％；采用Hha Ⅰ酶切检测广西巴马小型猪、巴长二元杂猪和长白猪氟烷基因的PCR产物,均产生2条电泳条带,未表现多态性；测序表明广西巴马小型猪氟烷基因第1 843位碱基均为C.猪氟烷基因PCR-RFLP分析技术为优质猪的培育提供了理论依据. |
| 关 键 词： | 广西巴马小型猪 氟烷基因 PCR-RFLP |
Analysis on Halothane Gene in Guangxi Bama Mini-pig with PCR-RFLP |
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| YAN Xue-yu , HUANG Yun , YAN Xiao-dong , JIANG Qing-yang , GUO Ya-fen , JIANG He-sheng. Analysis on Halothane Gene in Guangxi Bama Mini-pig with PCR-RFLP[J]. Hubei Agricultural Sciences, 2012, 51(18): 4144-4146 | |
| Authors: | YAN Xue-yu HUANG Yun YAN Xiao-dong JIANG Qing-yang GUO Ya-fen JIANG He-sheng |
| Affiliation: | 1(1.College of Animal Science and Technology of Guangxi University,Nanning 530004,China; 2.Animal Disease Prevention and Control Center of Fangchenggang,Fangchenggang 538021,Guangxi,China) |
| Abstract: | The genotype of halothane(Hal) gene in Guangxi Bama mini-pig was analysized by PCR-RFLP.The results showed that the length of the amplified fragment from mini-pig Hal gene was 452 bp,which had 99.6% homology with the reference sequence published in GenBank.Two electrophoresis bands were presented respectively when these fragments of Hal gene from Guangxi Bama mini-pigs,Bama mini-pig×Landrace crossbreds and Landraces were digested by HhaⅠ without polymorphism.The sequence results indicated that 1843rd base of all the mini-pig Hal gene were C.PCR-RFLP technique of porcine Hal gene could provide a theoretical basis for high-quality pig breeding. |
| Keywords: | Guangxi Bama mini-pig halothane gene PCR-RFLP |
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