| 植酸酶的酶学性质研究 |
| |
| 引用本文： | 王枫,韩志忠,董宏伟,杨成辉,杨雨虹.植酸酶的酶学性质研究[J].淡水渔业,2007,37(5):23-25. |
| |
| 作者姓名： | 王枫 韩志忠 董宏伟 杨成辉 杨雨虹 |
| |
| 作者单位： | 1. 东北农业大学动物科学技术学院水产养殖系,哈尔滨,150030
2. 哈尔滨市农业科学院水产分院,哈尔滨,150078 |
| |
| 基金项目： | 黑龙江省哈尔滨市科技攻关项目 |
| |
| 摘 要： | 以德国巴斯夫植酸酶为对象,研究了pH、温度以及热处理对其活性的影响。实验显示:该植酸酶在pH4.5～6的范围内均有80%以上的酶活性,最适pH为5.5;在温度30～50℃之间具有5 000 U/g以上的酶活性,最适温度为40℃;在80℃热处理20 min可保持90%以上的酶活,在90℃热处理20 min仅剩51.04%酶活。结果表明:pH、温度、热处理对植酸酶均有一定影响,饲料中添加植酸酶应该考虑这些因素。
|
| 关 键 词： | 酶活性 植酸酶 pH 温度 热稳定性 |
| 文章编号： | 1000-6907-（2007）05-0023-03 |
| 修稿时间： | 2007-03-13 |
A Study on Enzyme Characteristics of Phytase |
| |
| WANG Feng,HAN Zhi-zhong,DONG Hong-wei,YANG Cheng-hui,YANG Yu-hong.A Study on Enzyme Characteristics of Phytase[J].Freshwater Fisheries,2007,37(5):23-25. |
| |
| Authors: | WANG Feng HAN Zhi-zhong DONG Hong-wei YANG Cheng-hui YANG Yu-hong |
| |
| Institution: | 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030; 2. Fisheries Research Institute, Harbin Academy of Agricultural Sciences, Harbin 150078 |
| |
| Abstract: | Phytase from BASF was chosen as experimental material,its optimal pH,optimal temperature and thermal stability were studied respectively.In this trial,phytase had over 80% of the enzyme activity at pH 4.5～6,the optimal pH was 5.5.It had over 5000 U/g of the enzyme activity at the temperature of 30 ～50 ℃,the optimal temperature was 40 ℃.It could keep more than 90% of the enzyme activity when being subjected to 80 ℃ for 20 minutes.whereas the enzyme activity decreased to 51.04% when being subjected to 90 ℃ for 20 minutes.The results showed that pH,temperature and thermal stability should be considered when adding phytase to animal feed. |
| |
| Keywords: | pH |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
