Proteinase-activated receptor 2 expression in the intestinal tract of the horse |
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| Institution: | 1. Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany;2. State Office for Agriculture, Food Safety and Fishery Mecklenburg-Western Pommerania (LALLF M-V), Thierfelderstraße 18, 18059 Rostock, Germany;3. Clinic for Ruminants and Swine, Department of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany;1. Unidad Regional Universitaria Sursureste, Universidad Autónoma Chapingo, Km 7.5, carretera Teapa-Vicente Guerrero, P.O. Box 29, CP 86800, Teapa, Tabasco, México;2. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carr. Mérida-Xmatkuil km 15.5, CP 97100, Mérida, Yucatán, México;3. División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Teapa, km 25, CP 86280, Villahermosa, Tabasco, México;1. University College of London, Institute of Orhopaedics and Musculoskeletal Science, Royal National Orthopaedics Hospital, Brockley Hill, HA4 7LP, Stanmore, United Kingdom;2. Royal Veterinary College, Hawkshead Lane, North Mimms, AL9 7TA, Hatfield, United Kingdom;3. Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Distrito de Rubião Junior, S/N. Caixa Postal 560, CEP 18618-970, Botucatu, São Paulo-SP, Brazil;4. Veterinary Teaching Hospital, University of Brasilia, L4 Norte, CEP 70910-900, Brasilia-DF, Brazil;1. Laboratory of Carnivore Reproduction, Veterinary School, State University of Ceará, Fortaleza, Ceará, Brazil;2. VETER Diagnostic Center, Fortaleza, Ceará, Brazil;3. Faculdade de Tecnologia Intensiva, Fortaleza, Ceará, Brazil;4. Brazilian Army Force, Fortaleza, Ceará, Brazil;5. Graduate Program in Ecology and Natural Resources, Federal University of Ceará, Fortaleza, CE, Brazil;1. Department of Small Animals, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, UFSM. Avenida Roraima n° 1000, Camobi, Santa Maria, RS, CEP 97105-900, Brazil;2. Department of of Animal Science, Universidade do Estado de Santa Catarina, Rua Beloni Trombeta Zanin, 680-E. Bairro Santo Antônio, Chapecó, Santa Catarina, CEP 89815-630, Brazil;3. Department of Chemistry, Universidade Federal de Santa Maria, Avenida Roraima, Prédio 18 – Cidade Universitária; Faixa de Camobi, KM 9; Bairro Camobi; Santa Maria/RS, CEP 97105-900, Brazil;4. Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil;5. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Campus Universitário, Camobi – Santa Maria, Rio Grande do Sul, CEP 97105-000, Brazil;6. Department of Pathology, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, prédio 97B, UFSM – Avenida Roraima, n° 1000, Camobi, Santa Maria, Rio Grande do Sul, CEP 97105-900, Brazil |
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| Abstract: | Proteinase-activated receptor 2 (PAR2) is a G-protein-coupled receptor for trypsin and mast cell tryptase; it is highly expressed at the intestinal level with multiple functions, such as epithelial permeability and intestinal motility. Many proteases activate PAR2 during tissue damage, suggesting a role of the inflammatory response receptors. The aim of the study was to evaluate the distribution and expression of PAR2 in the jejunum, the ileum and the pelvic flexure, using samples collected from healthy adult horses after slaughter. Proteinase-activated receptor 2 immunoreactivity (PAR2-IR) was observed in the enterocytes, intestinal glands, the smooth muscle of the muscularis mucosae, and the longitudinal and circular muscle layers; there were no differences in the distribution of PAR2-IR in the different sections of the intestinal tract. The protein expression level showed that the relative amount of the PAR2 content in the mucosa of the intestinal tract decreased from the small to the large intestine while the PAR2 mRNA analysed showed similar values. This study provides relevant findings concerning the distribution of the PAR2 in the intestines of healthy horses and represents the starting point for evaluating the role of the PAR2 during strangulative intestinal disease and consequent systemic intestinal reperfusion/injury complications in horses in order to identify and employ antagonist PAR2 molecules. |
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