Wheat (Triticum aestivum L.) grain proteome response to elevated [CO2] varies between genotypes |
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| Institution: | 1. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, 4 Water Street, Creswick, Victoria 3363, Australia;2. Bio21 Molecular Science and Biotechnology Institute, Parkville, Victoria 3052 Australia;3. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia;4. Department of Economic Development, Jobs Transport and Resources, Horsham, Victoria 3400, Australia;5. Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Oura-gun, Gunma 374-0193 Japan;6. Centre for Crop Health, The University of Southern Queensland, Toowoomba, Queensland 4350, Australia;1. Plant Biology and Crop Science Department, Rothamsted Research, Harpenden AL5 4TX, UK;2. School of Ecosystem and Forest Sciences, The University of Melbourne, 4 Water Street, Creswick, VIC 3363, Australia;3. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville Campus, Melbourne, VIC 3010, Australia;4. Department of Economic Development, Jobs, Transport and Resources, 110 Natimuk Road, Horsham, VIC 3400, Australia |
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| Abstract: | The impact of rising carbon dioxide concentration (CO2]) in the atmosphere on wheat grain protein concentration and proteome was investigated in this study. Wheat genotypes (H45, SB003, SB062 and Yitpi) were grown in the Australian Grains Free-Air CO2 Enrichment (AGFACE) facility, Horsham, Victoria, Australia under ambient CO2] (aCO2], 391 μmol mol?1) and elevated CO2] (eCO2], 550 ± 20 μmol mol?1). Grain yield and grain protein concentration were measured. Global grain proteome comparison was carried out using stable isotope dimethyl labelling followed by liquid chromatography - mass spectrometry (LC-MS/MS). Grain yield was significantly increased at eCO2], whereas protein concentration was significantly decreased and responses varied between genotypes. Proteome-wide analysis revealed that protein composition was also altered under eCO2]. Grain protein concentration and composition of SB003 was very responsive to eCO2]. Mainly storage proteins were decreased at eCO2] and the responses varied between genotypes. These findings suggest that eCO2] may have a major impact on grain protein quality and thus bread quality and human and animal nutrition. Further, these findings suggest that CO2] insensitive cultivars can be identified for grain quality improvement under changing climate. |
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| Keywords: | Free-air carbon dioxide enrichment Wheat Protein Proteome |
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