Effect of particle size of rice bran on gamma-oryzanol content and compounds |
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| Institution: | 1. Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;2. Faculty of Science, Energy and Environment, King Mongkut’s University of Technology North Bangkok, Rayong Campus, Rayong 21120, Thailand;3. Division of Marine Product Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand;4. Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;1. Department of Lipid Science, CSIR-Central Food Technological Research Institute, Mysuru, 570020 Karnataka, India;2. Department of Diabetes, Endocrinology and Metabolism, University of Illinois at Chicago, 60607, USA;1. Department of Animal Science, Federal University of Pelotas, 96010-900 Pelotas, RS, Brazil;2. Department of Agroindustrial Science and Technology, Federal University of Pelotas, 96010-900 Pelotas, RS, Brazil;3. Brazilian Agricultural Research Corporation – Embrapa Swine and Poultry, Concórdia, SC, Brazil |
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| Abstract: | The reduction of particle size can facilitate the extraction of phytochemical compounds. In this study was evaluated the effect of rice bran particles size in the yield and characteristics of γ-oryzanol compound. The γ-oryzanol extraction was realized with hexane and isopropanol solvents and quantification by spectrophotometric method. The γ-oryzanol extracts were characterized in relation of theirs majority components in HPLC-UV and the antioxidant capacity verified by the free radical DPPH● consumption. The γ-oryzanol yield varied of 0.10–1.54 mg/g of bran, and the highest yield was obtained in particles smaller than 0.39 mm. The γ-oryzanol majority components presence (cycloartenyl ferulate, 2,4-methylenecycloartanyl ferulate, campesteryl ferulate e β-sitosteryl ferulate) in the extracts was confirmed and verified differences in the profile of this components in function of different particles sizes. The γ-oryzanol extract obtained from particle sizes between 0.73 and 1.67 mm demonstrated most specific inhibition of DPPH radical (6.7%) and IC50 6.63 μg/mL. When the particle size is reduced, the access surface to the extraction solvent is increased resulting in more γ-oryzanol extraction, however the extract from larger particles was more efficient as antioxidant. |
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| Keywords: | Antioxidant Extraction yield Inhibition Phytochemical |
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